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Infect Immun. 1970 March; 1(3): 259-262
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

research-article

Plaque Formation by Chlamydia in L Cells

Joyce Banks, B. Eddie, Julius Schachter and K. F. Meyer

1 The G. W. Hooper Foundation, University of California San Francisco Medical Center, San Francisco, California 94122

ABSTRACT

Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg LD50 determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-conjunctivitis agents did not produce plaques. The plaque assay is highly sensitive, reproducible, and offers a potential tool for investigations requiring accurate measurement of small changes in chlamydial infectivity.

Infect Immun. 1970 March; 1(3): 259-262
Copyright © 1970 American Society for Microbiology. All Rights Reserved.



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