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Infect Immun. 1970 May; 1(5): 446-454
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

research-article

Interconversion of Purine Mononucleotides in Pasteurella pestis¹

^a Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823

ABSTRACT

Included among the five established determinants of virulence in Pasteurella pestis are the abilities of cells to accomplish the de novo biosynthesis of purines and to grow as dark pigmented (P⁺) colonies on a solid synthetic medium containing hemin. P⁺ isolates of P. pestis strain KIM-10 (mouse intraperitoneal LD₅₀ < 10 cells) failed to convert exogenous guanine-8-¹⁴C to adenine residues of ribonucleic acid (RNA) when cultivated in a minimal medium which favored the pigmentation reaction. This conversion occurred in P⁺ cells grown in an enriched medium which did not support the pigmentation reaction and was observed in P^– mutants cultivated in both types of media. Both P⁺ and P^– isolates converted exogenous adenine-8-¹⁴C but not adenine-2-¹⁴C at a significant rate to guanosine residues of RNA when grown under a variety of conditions. This difference appeared to reflect a deficiency of adenine deaminase. The mouse intraperitoneal LD₅₀ of purine-auxotrophs was about 10² cells when the metabolic block occurred prior to the de novo formation of inosine monophosphate (IMP). In contrast, the corresponding value for a mutant blocked between IMP and guanine monophosphate was > 10⁷ cells in mice and > 10⁸ cells in guinea pigs.

¹ Presented in part at the 69th Annual Meeting of the American Society for Microbiology, Miami Beach, Fla., May 1969.

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