This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Binn, L. N. Articles by Kajima, M. Search for Related Content PubMed PubMed Citation Articles by Binn, L. N. Articles by Kajima, M.

 Previous Article  |  Next Article 

Infect Immun. 1970 May; 1(5): 503-508
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

research-article

Recovery and Characterization of a Minute Virus of Canines ¹

Department of Veterinary Microbiology, Walter Reed Army Institute of Research, Washington, D.C. 20012
Lobund Laboratories, University of Notre Dame, Notre Dame, Indiana 46556

ABSTRACT

Four antigenically related transmissible agents were recovered from canine fecal specimens. The agents produced cytopathic effects in a continuous dog cell line developed in this laboratory. Increased antibody titers were demonstrated in three of the four dogs which provided the isolates. The virus did not produce cytopathic effects in primary canine kidney or thymus cell cultures, or in cell cultures of human, simian, porcine, bovine, feline, and murine origin. The agent is resistant to ether, chloroform, and heat treatment, and the growth of the virus is inhibited by 5-iodo-2-deoxyuridine. After acridine orange staining, green fluorescent intranuclear inclusions are seen in infected cell cultures. By electron microscopy, the virions measure approximately 20 to 21 nm in overall diameter and are present in the nuclei of infected cells. These properties are consistent with membership in the parvovirus or picodnavirus group. The agent hemagglutinates rhesus red blood cells at 5 C and by hemagglutination-inhibition tests could be readily distinguished from H-1, rat virus, and the minute virus of mice. Canine gamma globulin contains high titers of neutralizing antibody and neutralizing antibody was found in a high percentage of military dogs and in beagles of a breeding colony.

² Present address: U.S. Military Group, Panama, U.S. Embassy, Box 2016, Balboa, Canal Zone.

¹ Presented in part before the American Society for Microbiology, Detroit, Mich., May 1968.

This article has been cited by other articles:

• Schildgen, O., Muller, A., Allander, T., Mackay, I. M., Volz, S., Kupfer, B., Simon, A. (2008). Human Bocavirus: Passenger or Pathogen in Acute Respiratory Tract Infections?. Clin. Microbiol. Rev. 21: 291-304 [Abstract] [Full Text]  
• Neske, F., Blessing, K., Tollmann, F., Schubert, J., Rethwilm, A., Kreth, H. W., Weissbrich, B. (2007). Real-Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis. J. Clin. Microbiol. 45: 2116-2122 [Abstract] [Full Text]  
• Ma, X., Endo, R., Ishiguro, N., Ebihara, T., Ishiko, H., Ariga, T., Kikuta, H. (2006). Detection of human bocavirus in Japanese children with lower respiratory tract infections.. J. Clin. Microbiol. 44: 1132-1134 [Abstract] [Full Text]  
• Url, A., Truyen, U., Rebel-Bauder, B., Weissenbock, H., Schmidt, P. (2003). Evidence of Parvovirus Replication in Cerebral Neurons of Cats. J. Clin. Microbiol. 41: 3801-3805 [Abstract] [Full Text]  
• Mochizuki, M., Hashimoto, M., Hajima, T., Takiguchi, M., Hashimoto, A., Une, Y., Roerink, F., Ohshima, T., Parrish, C. R., Carmichael, L. E. (2002). Virologic and Serologic Identification of Minute Virus of Canines (Canine Parvovirus Type 1) from Dogs in Japan. J. Clin. Microbiol. 40: 3993-3998 [Abstract] [Full Text]