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Infect Immun. 1975 May; 11(5): 991-995

Detection of virus-specific antibody-forming cells of mice immunized with Newcastle disease virus.

Y T Kim, J Ault and R J Wodzinski

ABSTRACT

The hemolytic plaque assay was adapted to the detection of antibodies to Newcastle disease virus (NDV) in an in vivo, an in vitro system, and a combined in vivo-in vitro system. Several conditions were tested for coupling of sheep erythrocytes to NDV and for the kinetics of plaque formation in the in vivo and in vitro systems. The one set of conditions which provided the best responses is presented. The effect of multiple injections of NDV into mice on plaque formation was optimized.

Infect Immun. 1975 May; 11(5): 991-995




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Copyright © 1975 by the American Society for Microbiology. All rights reserved.