This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shimonaka, H. Articles by Ito, Y. Search for Related Content PubMed PubMed Citation Articles by Shimonaka, H. Articles by Ito, Y.

Immunochemical Studies on the Human Pathogen Sporothrix schenckii: Effects of Chemical and Enzymatic Modification of the Antigenic Compounds upon Immediate and Delayed Reactions

Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu, Japan

ABSTRACT

The rhamnose-containing polysaccharide-peptide compound derived from the cells of the pathogenic fungus Sporothrix schenckii has been shown to contain 87.1% carbohydrate and 12.5% peptide and to give rise to both immediate- and delayed-type reactions in sensitized guinea pigs. The capacity to induce immediate-type reaction, passive cutaneous anaphylaxis, was completely lost by degradation of the carbohydrate moiety by periodate, whereas the ability to induce the delayed-type reactions of migration inhibition (in vitro) and the sporotrichin reaction (in vivo) were only slightly affected by periodate treatment. On the other hand, delayed "reactivities" to the compound were considerably reduced by treatment with papain, whereas the immediate-type reaction remained positive. These results lead to a conclusion that the rhamnose-containing polysaccharide of the polysaccharide-peptide antigenic compound plays an important role in the immediate-type reaction, whereas the peptide is largely responsible for the delayed-type reaction.