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Infect Immun. 1975 June; 11(6): 1195-1202
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Immunogenicity of Ribosomal Vaccines Isolated from Group A, Type 14 Streptococcus pyogenes

^a Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242

ABSTRACT

A ribosomal preparation isolated from group A, type 14 Streptococcus pyogenes protected mice against a challenge of 1,000 mean lethal doses. Optimal immunization consisted of subcutaneous administration of 326 µg of protein with Freund incomplete adjuvant. Animals immunized with higher or lower antigen concentrations showed decreased survival. Freund incomplete adjuvant was necessary to insure optimum immunization. Immunochemical studies showed no M protein or C carbohydrate present in the ribosomal preparation. Mice optimally immunized with the type 14 ribosomal preparation were protected against heterologous challenge with M types 2, 5, 8, and 12. Protein-free ribonucleic acid extracts isolated from the type 14 ribosomal preparation did not protect mice against homologous challenge. But ribonucleic acid-free protein extracts showed 100% protection in mice after homologous challenge.

¹ Present address: Center for Disease Control, Atlanta, Ga. 30333.