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Infect Immun. 1975 June; 11(6): 1203-1209
Copyright © 1975 American Society for Microbiology. All Rights Reserved.

Immunological Properties of Rickettsia rickettsii Purified by Zonal Centrifugation

R. L. Anacker, R. K. Gerloff, L. A. Thomas, R. E. Mann and W. D. Bickel

Rocky Mountain Laboratory, Hamilton, Montana 59840

ABSTRACT

The properties of Rickettsia rickettsii purified from infected chicken yolk sacs or mouse L cell cultures by sucrose density gradient centrifugation in a zonal rotor were examined in various ways. Rickettsiae derived from both L cells and yolk sacs had similar compositions: about 12% nitrogen, 1.5% phosphorus, 5% carbohydrate, and 30% fatty acids. On a dry-weight basis, purified rickettsiae were at least 2,000 times as effective as a commercial spotted fever vaccine in protecting guinea pigs against infection with spotted fever rickettsiae and mice against death from toxin of R. rickettsii. Gradient-purified rickettsiae (0.6 µg) induced a serological response in guinea pigs of the same magnitude as that stimulated by 1,600 µg of the commercial vaccine. Gradient-purified rickettsiae had little group reactivity in complement fixation tests but became anti-complementary upon storage. Microagglutination and hemagglutination tests with the purified antigen gave promise of usefulness in diagnosis of spotted fever. These results suggest that zonal centrifugation may be a valuable procedure for the preparation of R. rickettsii vaccine and diagnostic reagent.

Infect Immun. 1975 June; 11(6): 1203-1209
Copyright © 1975 American Society for Microbiology. All Rights Reserved.



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Copyright © 1975 by the American Society for Microbiology. All rights reserved.