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Infect Immun. 1975 August; 12(2): 270-273

Cellular location of alpha-hemolysin in Staphylococcus aureus.

K M Nugent, G B Calandra and T S Theodore

ABSTRACT

Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.

Infect Immun. 1975 August; 12(2): 270-273






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