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Measurement of biological activities of purified and crude enterotoxin of Clostridium perfringens.

ABSTRACT

Enterotoxin of Clostridium perfringens was assayed and compared with toxicity in mice and erythemal activity in guinea pigs. Conversion factors were used to express these biological activities of crude enterotoxin in terms of weight of pure enterotoxin protein. One microgram of enterotoxin was equivalent to 3.41 erythema units and to 0.68 mouse median lethal dose.

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