This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reiter, B Articles by Rosén, C G Search for Related Content PubMed PubMed Citation Articles by Reiter, B Articles by Rosén, C G

 Previous Article  |  Next Article 

Infect Immun. 1976 March; 13(3): 800-807

Nonspecific bactericidal activity of the lactoperoxidases-thiocyanate-hydrogen peroxide system of milk against Escherichia coli and some gram-negative pathogens.

ABSTRACT

Two strains of Escherichia coli and one strain each of Salmonella typhimurium and Pseudomonas aeruginosa were killed by the bactericidal activity of the lactoperoxidase-thiocyanate-hydrogen peroxide system in milk and in a synthetic medium. H2O2 was supplied exogenously by glucose oxidase, and glucose was produced at a level which was itself noninhibitory. Two phases were distinguished: the first phase was dependent on the oxidation of SCN(-) by lactoperoxidase and H2O2, which was reversed by reducing agent, and the second phase was dependent on the presence of accumulated H2O2, which was reversed by catalase. The latter enzyme could also reverse the first phase, but only when present in excessive and unphysiological levels. The bactericidal activity was greatest at pH 5 and below, and it depended on the SCN(-)concentration and on the number of organisms. Since raw or heated milk neutralizes the acid barrier against infection in the stomach, the bactericidal system discussed may contribute to the prevention of enteric infections in neonates.

This article has been cited by other articles:

• Wijkstrom-Frei, C., El-Chemaly, S., Ali-Rachedi, R., Gerson, C., Cobas, M. A., Forteza, R., Salathe, M., Conner, G. E. (2003). Lactoperoxidase and Human Airway Host Defense. Am. J. Respir. Cell Mol. Bio. 29: 206-212 [Abstract] [Full Text]  
• Adak, S., Mazumdar, A., Banerjee, R. K. (1997). Low Catalytic Turnover of Horseradish Peroxidase in Thiocyanate Oxidation. EVIDENCE FOR CONCURRENT INACTIVATION BY CYANIDE GENERATED THROUGH ONE-ELECTRON OXIDATION OF THIOCYANATE. J. Biol. Chem. 272: 11049-11056 [Abstract] [Full Text]