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Infect Immun. 1976 September; 14(3): 623-625

Isolation of a purified skin test antigen from Blastomyces dermatitidis yeast-phase cell wall.

M V Lancaster and R F Sprouse

ABSTRACT

Preparative polyacrylamide gel electrophoresis was used to isolate individual components of an alkaline-soluble-water-soluble fraction of the cell wall of Blastomyces dermatitidis yeast phase. One component isolated demonstrated exceptional specificity and reactivity when tested on guinea pigs infected with B. dermatitidis. This component displayed no cross-reactivity when tested on animals infected with Histoplasma capsulatum. The significance of isolation of a purified, specific antigen is discussed.

Infect Immun. 1976 September; 14(3): 623-625






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