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Infect Immun. 1976 October; 14(4): 1028-1033

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

T Honda, M Shimizu, Y Takeda and T Miwatani

ABSTRACT

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-Sephadex A-25, hydroxylapatite, and Sephadex G-200. This factor was separated from thermostabl direct hemolysin and was heat labile.

Infect Immun. 1976 October; 14(4): 1028-1033




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