This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cherniak, R Articles by Frederick, H M Search for Related Content PubMed PubMed Citation Articles by Cherniak, R Articles by Frederick, H M

 Previous Article  |  Next Article 

Infect Immun. 1977 March; 15(3): 765-771

Capsular polysaccharide of Clostridium perfringens Hobbs 9.

ABSTRACT

Several closely related capsular polysaccharides were isolated from a strain of Clostridium perfringens Hobbs 9 type A by extraction of encapsulated cells with cold 0.85% NaCl. The soluble polymers were precipitated with alcohol and purified by (NH4)2SO4 fractionation, enzymatic digestion with papain and ribonuclease, and chromatography on diethylaminoethyl-Sephadex A25. The polysaccharides were composed mainly of glucose, galactose, and galactosamine. The major fraction contained these constituents (representing 77% of the dry weight) in a molar ratio of 1:1.6:1.1. All of the fractions contained phosphate and peptide material that was not removed during purification. The polysaccharides were closely related but not identical as indicated by double-diffusion-in-gel experiments. Immunoelectrophoresis in agarose demonstrated that the polysaccharides had identical mobilities and that no resolution into additional fractions occurred. The immunological activity of all the purified polysaccharides was destroyed by periodate oxidation but was unaffected by protease.

This article has been cited by other articles:

• Varga, J., Stirewalt, V. L., Melville, S. B. (2004). The CcpA Protein Is Necessary for Efficient Sporulation and Enterotoxin Gene (cpe) Regulation in Clostridium perfringens. J. Bacteriol. 186: 5221-5229 [Abstract] [Full Text]  
• O'Brien, D. K., Melville, S. B. (2003). Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors. Microbiology 149: 1377-1386 [Abstract] [Full Text]  
• Walters, D. M., Stirewalt, V. L., Melville, S. B. (1999). Cloning, Sequence, and Transcriptional Regulation of the Operon Encoding a Putative N-Acetylmannosamine-6-Phosphate Epimerase (nanE) and Sialic Acid Lyase (nanA) in Clostridium perfringens. J. Bacteriol. 181: 4526-4532 [Abstract] [Full Text]