This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clark, W. B.
Right arrow Articles by Gibbons, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clark, W. B.
Right arrow Articles by Gibbons, R. J.

 Previous Article  |  Next Article 

Infect Immun. 1977 November; 18(2): 514-523
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Influence of Salivary Components and Extracellular Polysaccharide Synthesis from Sucrose on the Attachment of Streptococcus mutans 6715 to Hydroxyapatite Surfaces

W. B. Clark and R. J. Gibbons

1 Forsyth Dental Center, Harvard School of Dental Medicine, Boston, Massachusetts 02115

ABSTRACT

The adsorption of 3H-labeled Streptococcus mutans 6715 cells to disks of hydroxyapatite (HA) was studied. The number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 x 108 per ml; equilibrium occurred within 45 min. Assay reliability was verified by direct scanning electron microscopic counts. Untreated HA disks exposed to buffered saline (PBS)-suspended streptococci at a concentration of 1.1 x 108 per ml absorbed 3.2 x 106 cells per cm2; approximately 3% of the surface area was, therefore, occupied by adsorbed organisms. The presence of adsorbed salivary components on HA reduced the number of attaching S. mutans cells by half. When S. mutans cells were suspended in saliva to mimic conditions existing in the mouth, the number of streptococci adsorbing to saliva-treated HA was reduced more than 30-fold compared to untreated HA. Approximately one-half of the streptococci adsorbed to untreated or to saliva-treated HA disks could be desorbed over a 4-h period with 0.067 M phosphate buffer. S. mutans cells exposed to sucrose to permit extracellular polysaccharide synthesis before or during adsorption attached in fewer numbers to both saliva-treated and untreated HA than PBS-treated organisms. When S. mutans cells adsorbed on untreated HA were exposed to sucrose, fewer organisms could be desorbed; thus, in situ polysaccharide synthesis promoted their more firm attachment to untreated HA. However, when saliva-suspended streptococci were adsorbed to saliva-treated HA surfaces, exposure to sucrose before or subsequent to adsorption did not promote more firm attachment. Evidently, the powerful adherence-inhibiting and desorptive effects of salivary components overshadowed any promoting effects attributable to glucan synthesis from sucrose. Similarly, no differences were noted in the desorption of S. mutans cells from human teeth after exposure to sucrose, glucose, or PBS relative to a strain of Streptococcus mitis (S. mitior). Thus, no evidence was obtained to support the hypothesis that glucan synthesis from sucrose was essential for, or promoted, the attachment of S. mutans cells to HA surfaces exposed to saliva or to the smooth surfaces of human teeth.

Infect Immun. 1977 November; 18(2): 514-523
Copyright © 1977 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gyo, M., Nikaido, T., Okada, K., Yamauchi, J., Tagami, J., Matin, K. (2008). Surface Response of Fluorine Polymer-Incorporated Resin Composites to Cariogenic Biofilm Adherence. Appl. Environ. Microbiol. 74: 1428-1435 [Abstract] [Full Text]  
  • Chatfield, C. H., Koo, H., Quivey,, R. G. Jr (2005). The putative autolysin regulator LytR in Streptococcus mutans plays a role in cell division and is growth-phase regulated. Microbiology 151: 625-631 [Abstract] [Full Text]  
  • Mombelli, A. (1999). In Vitro Models of Biological Responses to Implant Microbiological Models. ADR 13: 67-72 [Abstract]  
  • Schupbach, P., Neeser, J.R., Golliard, M., Rouvet, M., Guggenheim, B. (1996). Incorporation of Caseinoglycomacropeptide and Caseinophosphopeptide into the Salivary Pellicle Inhibits Adherence of Mutans Streptococci. JDR 75: 1779-1788 [Abstract]  
  • Scannapieco, F.A., Torres, G.I., Levine, M.J. (1995). Salivary Amylase Promotes Adhesion of Oral Streptococci to Hydroxyapatite. JDR 74: 1360-1366 [Abstract]  
  • Scannapieco, F. A. (1994). Saliva-Bacterium Interactions in Oral Microbial Ecology. CROBM 5: 203-248 [Abstract] [Full Text]  
  • Scannapieco, F. A., Torres, G., Levine, M. J. (1993). Salivary {alpha}-Amylase: Role in Dental Plaque and Caries Formation. CROBM 4: 301-307 [Abstract] [Full Text]  
  • Weerkamp, A.H., Van der Mei, H.C., Busscher, H.J. (1985). The Surface Free Energy of Oral Streptococci after being Coated with Saliva and its Relation to Adhesion in the Mouth. JDR 64: 1204-1210 [Abstract]  
  • Gibbons, R.J., Etherden, I., Moreno, E.C. (1985). Contribution of Stereochemical Interactions in the Adhesion of Streptococcus sanguis C5 to Experimental Pellicles. JDR 64: 96-101 [Abstract]  
  • Stinson, M.W., Levine, M.J., Cavese, J.M., Prakobphol, A., Murray, P.A., Tabak, L.A., Reddy, M.S. (1982). Clinical Science: Adherence of Streptococcus sanguis to Salivary Mucin Bound to Glass. JDR 61: 1390-1393 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»