This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hejna, J Articles by Cameron, J A Search for Related Content PubMed PubMed Citation Articles by Hejna, J Articles by Cameron, J A

 Previous Article  |  Next Article 

Infect Immun. 1978 January; 19(1): 187-193

Effect on particle size of solubilization of wild-type and Re chemotype lipopolysaccharides solubilized with bovine serum albumin and triethylamine.

ABSTRACT

Disaggregation of wild-type and Re chemotype lipopolysaccharides (LPS) has been accomplished by solubilization with triethylamine followed by stabilization with bovine serum albumin in order to determine the degree of aggregation. Solubilization with 1.5% triethylamine was found to be more effective than with lower concentrations, as judged by permeation chromatography. Chromatographic analysis of triethylamine-bovine serum albumin-disaggregated LPS showed a range of particle sizes. Wild-type LPS yielded size classes of 1 X 10(6) to 4 X 10(6) daltons, 250,000 daltons, and 20,000 daltons. Re LPS yielded size classes of 1 X 10(6) to 4 X 10(6) daltons and 20,000 daltons. Untreated LPS was always greater than 4 X 10(6) daltons. Chemical characterization of the size classes revealed 3-keto-2-deoxyoctonate in all size classes and suggests that the O-antigenic side chain length may determine the aggregate size in solubilized LPS.