This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Slots, J.
Right arrow Articles by Gibbons, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slots, J.
Right arrow Articles by Gibbons, R. J.

 Previous Article  |  Next Article 

Infect Immun. 1978 January; 19(1): 254-264
Copyright © 1978 American Society for Microbiology. All Rights Reserved.

Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to Oral Surfaces and Its Possible Role in Colonization of the Mouth and of Periodontal Pockets

Jörgen Slots and Ronald J. Gibbons

1 Forsyth Dental Center, Boston, Massachusetts 02115

ABSTRACT

This investigation examined the ability of cells of Bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. Of 48 asaccharolytic strains of B. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. Electron microscopy indicated that both asaccharolytic and fermentative strains possessed pili; hence, the presence of pili did not correlate with the hemagglutinating activities of B. melaninogenicus strains. Both asaccharolytic and fermentative B. melaninogenicus strains suspended in phosphate-buffered saline adhered in high numbers to buccal epithelial cells and to the surfaces of several gram-positive bacteria tested, including Actinomyces viscosus, A. naeslundii, A. israelii, Streptococcus sanguis, and S. mitis. B. melaninogenicus subsp. asaccharolyticus 381 also attached, but in comparatively low numbers, to untreated and to saliva-treated hydroxyapatite. Addition of clarified whole saliva to suspensions of strain 381 almost completely eliminated adherence to buccal epithelial cells and to hydroxyapatite surfaces, but saliva had no detectable effect on attachment to gram-positive plaque bacteria. Both fermentative and nonfermentative strains of B. melaninogenicus also attached in high numbers to crevicular epithelial cells derived from human periodontal pockets, but normal human serum strongly inhibited attachment. Serum also inhibited attachment of strain 381 to saliva- and serum-treated hydroxyapatite, but it had little effect upon attachment to gram-positive bacteria. These observations suggested that salivary and serum components would strongly inhibit the attachment of B. melaninogenicus cells to several oral surfaces, but not to the surfaces of certain gram-positive bacteria commonly present in human dental plaque. This was confirmed by an in vivo experiment in which streptomycin-labeled cells of B. melaninogenicus 381-R were introduced into the mouths of two volunteers. After 10 min, several hundred-fold higher numbers of the organism were recovered from preformed bacterial plaque present on teeth than from clean tooth surfaces or from the buccal mucosa and tongue dorsum. High numbers of B. melaninogenicus cells were also recovered from preformed plaque after 150 min, but virtually no cells of the organism were recovered from the other surfaces studied. These data suggest that the presence of dental plaque containing Actinomyces and other gram-positive bacteria may be essential for the attachment and colonization of B. melaninogenicus cells after their initial introduction into the mouth. Similarly, the presence of subgingival plaque containing gram-positive bacteria may be necessary for its secondary colonization in periodontal pockets.

Infect Immun. 1978 January; 19(1): 254-264
Copyright © 1978 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Enersen, M., Olsen, I., Kvalheim, O., Caugant, D. A. (2008). fimA Genotypes and Multilocus Sequence Types of Porphyromonas gingivalis from Patients with Periodontitis. J. Clin. Microbiol. 46: 31-42 [Abstract] [Full Text]  
  • Kuramitsu, H. K., He, X., Lux, R., Anderson, M. H., Shi, W. (2007). Interspecies Interactions within Oral Microbial Communities. Microbiol. Mol. Biol. Rev. 71: 653-670 [Abstract] [Full Text]  
  • Sakai, E., Naito, M., Sato, K., Hotokezaka, H., Kadowaki, T., Kamaguchi, A., Yamamoto, K., Okamoto, K., Nakayama, K. (2007). Construction of Recombinant Hemagglutinin Derived from the Gingipain-Encoding Gene of Porphyromonas gingivalis, Identification of Its Target Protein on Erythrocytes, and Inhibition of Hemagglutination by an Interdomain Regional Peptide. J. Bacteriol. 189: 3977-3986 [Abstract] [Full Text]  
  • Simionato, M. R., Tucker, C. M., Kuboniwa, M., Lamont, G., Demuth, D. R., Tribble, G. D., Lamont, R. J. (2006). Porphyromonas gingivalis Genes Involved in Community Development with Streptococcus gordonii. Infect. Immun. 74: 6419-6428 [Abstract] [Full Text]  
  • Maeda, K., Nagata, H., Kuboniwa, M., Kataoka, K., Nishida, N., Tanaka, M., Shizukuishi, S. (2004). Characterization of Binding of Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase to Porphyromonas gingivalis Major Fimbriae. Infect. Immun. 72: 5475-5477 [Abstract] [Full Text]  
  • Maeda, K., Nagata, H., Yamamoto, Y., Tanaka, M., Tanaka, J., Minamino, N., Shizukuishi, S. (2004). Glyceraldehyde-3-Phosphate Dehydrogenase of Streptococcus oralis Functions as a Coadhesin for Porphyromonas gingivalis Major Fimbriae. Infect. Immun. 72: 1341-1348 [Abstract] [Full Text]  
  • Kamaguchi, A., Ohyama, T., Sakai, E., Nakamura, R., Watanabe, T., Baba, H., Nakayama, K. (2003). Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia. Microbiology 149: 1257-1264 [Abstract] [Full Text]  
  • Demuth, D. R., Irvine, D. C., Costerton, J. W., Cook, G. S., Lamont, R. J. (2001). Discrete Protein Determinant Directs the Species-Specific Adherence of Porphyromonas gingivalis to Oral Streptococci. Infect. Immun. 69: 5736-5741 [Abstract] [Full Text]  
  • Chung, W. O., Demuth, D. R., Lamont, R. J. (2000). Identification of a Porphyromonas gingivalis Receptor for the Streptococcus gordonii SspB Protein. Infect. Immun. 68: 6758-6762 [Abstract] [Full Text]  
  • Abiko, Y. (2000). Passive Immunization Against Dental Caries and Periodontal Disease: Development of Recombinant and Human Monoclonal Antibodies. CROBM 11: 140-158 [Abstract] [Full Text]  
  • Shi, Y., Ratnayake, D. B., Okamoto, K., Abe, N., Yamamoto, K., Nakayama, K. (1999). Genetic Analyses of Proteolysis, Hemoglobin Binding, and Hemagglutination of Porphyromonas gingivalis. CONSTRUCTION OF MUTANTS WITH A COMBINATION OF rgpA, rgpB, kgp, AND hagA. J. Biol. Chem. 274: 17955-17960 [Abstract] [Full Text]  
  • Shibata, Y., Hayakawa, M., Takiguchi, H., Shiroza, T., Abiko, Y. (1999). Determination and Characterization of the Hemagglutinin-associated Short Motifs Found in Porphyromonas gingivalis Multiple Gene Products. J. Biol. Chem. 274: 5012-5020 [Abstract] [Full Text]  
  • Lamont, R. J., Jenkinson, H. F. (1998). Life Below the Gum Line: Pathogenic Mechanisms of Porphyromonas gingivalis. Microbiol. Mol. Biol. Rev. 62: 1244-1263 [Abstract] [Full Text]  
  • Kadowaki, T., Nakayama, K., Yoshimura, F., Okamoto, K., Abe, N., Yamamoto, K. (1998). Arg-gingipain Acts as a Major Processing Enzyme for Various Cell Surface Proteins in Porphyromonas gingivalis. J. Biol. Chem. 273: 29072-29076 [Abstract] [Full Text]  
  • Jenkinson, H.F., Lamont, R. (1997). Streptococcal Adhesion and Colonization. CROBM 8: 175-200 [Abstract] [Full Text]  
  • Liljemark, W.F., Bloomquist, C. (1996). Human Oral Microbial Ecology and Dental Caries and Periodontal Diseases. CROBM 7: 180-198 [Abstract] [Full Text]  
  • Stinson, M. W., Levine, M. J. (1993). Modulation of Intergeneric Adhesion of Oral Bacteria by Human Saliva. CROBM 4: 309-314 [Full Text]  
  • Cowan, M.M., Van der Mei, H.C., Stokroos, I., Busscher, H.J. (1992). Heterogeneity of Surfaces of Subgingival Bacteria as Detected by Zeta Potential Measurements. JDR 71: 1803-1806 [Abstract]  
  • Loos, B.G., Dyer, D.W. (1992). Restriction Fragment Length Polymorphism Analysis of the Fimbrillin Locus, fimA, of Porphyromonas gingivalis. JDR 71: 1173-1181 [Abstract]  
  • Li, J., Ellen, R.P., Hoover, C.I., Felton, J.R. (1991). Association of Proteases of Porphyromonas (Bacteroides) gingivalis with its Adhesion to Actinomyces viscosus. JDR 70: 82-86 [Abstract]  
  • Nagata, H., Murakami, Y., Inoshita, E., Shizukuishi, S., Tsunemitsu, A. (1990). Inhibitory Effect of Human Plasma and Saliva on Co-aggregation Between Bacteroides gingivalis and Streptococcus mitis. JDR 69: 1476-1479 [Abstract]  
  • Loos, B.G., Mayrand, D., Genco, R.J., Dickinson, D.P. (1990). Genetic Heterogeneity of Porphyromonas (Bacteroides) gingivalis by Genomic DNA Fingerprinting. JDR 69: 1488-1493 [Abstract]  
  • Li, J., Ellen, R.P. (1989). Relative Adherence of Bacteroides Species and Strains to Actinomyces viscosus on Saliva-coated Hydroxyapatite. JDR 68: 1308-1312 [Abstract]  
  • Liljemarki, W.F., Bloomquist, C.G., Coulter, M.C., Fenner, L.J., Skopek, R.J., Schachtele, C.F. (1988). Utilization of a Continuous Streptococcal Surface to Measure Interbacterial Adherence in vitro and in vivo. JDR 67: 1455-1460 [Abstract]  
  • Suzuki, Y., Yoshimura, F., Tani, H., Suzuki, T. (1988). Fimbriae From the Oral Anaerobe Bacteroides Gingivalis: A Screening of Clinical Isolates From Various Places. ADR 2: 301-303 [Abstract]  
  • Gibbons, R.J. (1984). Microbial Ecology Adherent Interactions Which May Affect Microbial Ecology in the Mouth. JDR 63: 378-385 [Abstract]  
  • Cisar, J.O., Sandberg, A.L., Mergenhagen, S.E. (1984). The Function and Distribution of Different Fimbriae on Strains of Actinomyces viscosus and Actinomyces naeslundii. JDR 63: 393-396 [Abstract]  
  • Slots, J., Genco, R.J. (1984). Microbial Pathogenicity Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Virulence Factors in Colonization, Survival, and Tissue Destruction. JDR 63: 412-421  
  • Takazoe, I., Nakamura, T., Okuda, K. (1984). Colonization of the Subgingival Area by Bacteroides gingivalis. JDR 63: 422-426  
  • Siegrist, B., Kornman, K.S. (1982). The Effect of Supragingival Plaque Control on the Composition of the Subgingival Microbial Flora in Ligature-induced Periodontitis in the Monkey. JDR 61: 936-941 [Abstract]  
  • Aufdemorte, T.B., Cameron, I. L. (1981). The Relation of Keratinization to Bacterial Colonization on the Baboon Tongue as Demonstrated by Scanning Electron Microscopy. JDR 60: 1008-1014 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»