This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wyrick, P B
Right arrow Articles by Brownridge, E A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wyrick, P B
Right arrow Articles by Brownridge, E A

 Previous Article  |  Next Article 

Infect Immun. 1978 March; 19(3): 1054-1060

Growth of Chlamydia psittaci in macrophages.

P B Wyrick and E A Brownridge

ABSTRACT

Survival and growth of L-cell-cultivated Chlamydia psittaci occurred in mouse macrophages in vitro. Two major factors governing the intracellular fate of chlamydiae in macrophages are: (i) the multiplicity of infection (MOI), i.e., the elementary body (EB)-to-macrophage ratio, and (ii) the state of the EB. At a low MOI (1:1) survival and growth of live, untreated chlamydiae were optimal. The chlamydiae were internalized in macrophages within 30 to 40 min. EB proceeded to differentiate into reticulate bodies, which underwent multiplication and further matured into infectious EB in the professional phagocytic cells. In contrast, at a high MOI (100:1), survival of untreated chlamydiae was greatly reduced as a result of immediate damage to the macrophages. eb that were pretreated with heat (56 degrees C for 10 to 30 min) or coated with homologous antibody were rapidly destroyed in macrophage phagolysosomes. Fusion of ferritin-labeled lysosomes with heat-treated or opsonized EB-laden phagosomes occurred in 2 to 4 h, resulting in transfer of the ferritin marker into phagolysosomes.

Infect Immun. 1978 March; 19(3): 1054-1060




This article has been cited by other articles:

  • Prantner, D., Darville, T., Sikes, J. D., Andrews, C. W. Jr., Brade, H., Rank, R. G., Nagarajan, U. M. (2009). Critical Role for Interleukin-1{beta} (IL-1{beta}) during Chlamydia muridarum Genital Infection and Bacterial Replication-Independent Secretion of IL-1{beta} in Mouse Macrophages. Infect. Immun. 77: 5334-5346 [Abstract] [Full Text]  
  • Clemens, D. L., Lee, B.-Y., Horwitz, M. A. (2004). Virulent and Avirulent Strains of Francisella tularensis Prevent Acidification and Maturation of Their Phagosomes and Escape into the Cytoplasm in Human Macrophages. Infect. Immun. 72: 3204-3217 [Abstract] [Full Text]  
  • Scidmore, M. A., Fischer, E. R., Hackstadt, T. (2003). Restricted Fusion of Chlamydia trachomatis Vesicles with Endocytic Compartments during the Initial Stages of Infection. Infect. Immun. 71: 973-984 [Abstract] [Full Text]  
  • Clemens, D. L., Lee, B.-Y., Horwitz, M. A. (2002). The Mycobacterium tuberculosis Phagosome in Human Macrophages Is Isolated from the Host Cell Cytoplasm. Infect. Immun. 70: 5800-5807 [Abstract] [Full Text]  
  • Greub, G., Raoult, D. (2002). Crescent Bodies of Parachlamydia acanthamoeba and Its Life Cycle within Acanthamoeba polyphaga: an Electron Micrograph Study. Appl. Environ. Microbiol. 68: 3076-3084 [Abstract] [Full Text]  
  • Parchure, N., Zouridakis, E. G., Kaski, J. C. (2002). Effect of Azithromycin Treatment on Endothelial Function in Patients With Coronary Artery Disease and Evidence of Chlamydia pneumoniae Infection. Circulation 105: 1298-1303 [Abstract] [Full Text]  
  • Ericson, K., Saldeen, T. G. P., Lindquist, O., Pahlson, C., Mehta, J. L. (2000). Relationship of Chlamydia pneumoniae Infection to Severity of Human Coronary Atherosclerosis. Circulation 101: 2568-2571 [Abstract] [Full Text]  
  • Howe, D., Mallavia, L. P. (1999). Coxiella burnetii Infection Increases Transferrin Receptors on J774A.1 Cells. Infect. Immun. 67: 3236-3241 [Abstract] [Full Text]  
  • Majeed, M, Krause, K., Clark, R., Kihlstrom, E, Stendahl, O (1999). Localization of intracellular Ca2+ stores in HeLa cells during infection with Chlamydia trachomatis. J. Cell Sci. 112: 35-44 [Abstract]  
  • Bannantine, J. P., Stamm, W. E., Suchland, R. J., Rockey, D. D. (1998). Chlamydia trachomatis IncA Is Localized to the Inclusion Membrane and Is Recognized by Antisera from Infected Humans and Primates. Infect. Immun. 66: 6017-6021 [Abstract] [Full Text]  
  • Rittig, M. G., Schroppel, K., Seack, K.-H., Sander, U., N'Diaye, E.-N., Maridonneau-Parini, I., Solbach, W., Bogdan, C. (1998). Coiling Phagocytosis of Trypanosomatids and Fungal Cells. Infect. Immun. 66: 4331-4339 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»