This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Herman-Brand, R Articles by Sultzer, B M Search for Related Content PubMed PubMed Citation Articles by Herman-Brand, R Articles by Sultzer, B M

Inhibition by human serum of lymphocyte proliferation stimulated by purified protein derivative of tuberculin and bacillus Calmette-Guérin.

ABSTRACT

The addition of normal human serum to murine lymphocyte cultures consistently depressed mitogen-induced transformation, as measured by deoxyribonucleic acid synthesis. Stimulation by the B-cell mitogens purified protein derivative-tuberculin, bacillus Calmette-Guérin, and lipopolysaccharide was consistently affected, but there was no inhibition of T-cells when human serum was added to concanavalin A-stimulated cultures. The inhibitory effects were not due to cytotoxic factors for B-lymphocytes or to specific antibodies in serum directed against the mitogens. Analogous results were found with guinea pig serum. Contact of the lymphocytes with the serum within the first 24 h of culture was necessary for inhibition.