This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services E-mail this article to a friend Similar articles in this journal Similar articles in ASM journals Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lynn, R J Articles by Smith, P F Search for Related Content PubMed PubMed Citation Articles by Lynn, R J Articles by Smith, P F

 Previous Article  |  Next Article 

Infect Immun. 1980 September; 29(3): 926-933

Humoral immune response of rabbits to acholeplasmal lipoglycans.

R J Lynn, P A Guidici, E J Hollemweguer and P F Smith

ABSTRACT

Lipoglycans extracted from Acholeplasma species with hot aqueous phenol were immunogenic for rabbits when introduced by an appropriate method. All lipoglycans examined elicited antibody associated with a heavy, 2-mercaptoethanol-sensitive immunoglobulin fraction when inoculated intravenously adsorbed to autologous rabbit erythrocytes. This antibody was specific for the Acholeplasma species from which the lipoglycan was extracted. Extensive immunization of these animals with acholeplasmal lipoglycans produced significant increases in sheep erythrocyte hemolysin. Some, but not all, Acholeplasma species yielded lipoglycans that were immunogenic when emulsified with Freund complete adjuvant and introduced via the footpad into rabbits. Such animals produced antibodies corresponding to the M and G immunoglobulin classes that reacted with both homologous and heterologous acholeplasmal lipoglycans by precipitation in immunodiffusion as well as passive hemagglutination. None of the animals inoculated demonstrated a significant anamnestic response after booster injections either intravenously or via the footpads.

---

Infect Immun. 1980 September; 29(3): 926-933