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Infect Immun. 1971 February; 3(2): 243-248
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen

^a Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840

ABSTRACT

Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing most of the agglutinogen 1 were free of detectable mouse-protecting or histamine-sensitizing activities. Agglutinogen 1 from a serotype 1, 3 B. pertussis strain (J20) migrated similarly to the agglutinogen 1 from strain 353/Z. All agglutinogen 3 activity was found at the point of application in the starch block. No clear relationship was found between agglutinogen 1 and mouse-protecting antigen or histamine-sensitizing factor.

¹ Present address: Associate Professor, Veterinary Medical Research Institute, Iowa State University, Ames, Iowa 50010.