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Infect Immun. 1971 February; 3(2): 328-332
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Effect of L-Homoserine on the Growth of Mycobacterium tuberculosis

Thomas P. O'Barr and Karen A. Everett

Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240

ABSTRACT

L-Homoserine was observed to inhibit the growth of Mycobacterium tuberculosis. In the metabolism of M. tuberculosis, L-homoserine was found to be a precursor of threonine, isoleucine, and methionine. L-Homoserine-inhibited cells contained elevated levels of the enzyme acetohydroxy acid synthetase. In addition, washed cell suspensions of M. tuberculosis formed significant amounts of alpha-amino-n-butyric acid from supplements of L-homoserine. DL-Alpha-amino-n-butyric acid proved to be much more inhibitory for growth than L-homoserine. Growth antagonism by L-homoserine was reversed by L-lysine, L-threonine, and combinations of L-leucine with L-valine. At the cellular level, these amino acids reduced the amount of acetohydroxy acid synthetase in cells grown with L-homoserine and competed with DL-homoserine-4-14C for entrance into the extractable cell pool. L-Isoleucine also antagonized the conversion of L-homoserine to alpha-amino-n-butyric acid. Available data indicated that the effect of L-homoserine was related to its conversion to alpha-amino-n-butyric acid which subsequently inhibited growth

Infect Immun. 1971 February; 3(2): 328-332
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



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