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Metabolic Event Involved in the Bactericidal Activity of Normal Mouse Macrophages

¹ Trudeau Institute, Saranac Lake, New York 12983

ABSTRACT

The intracellular bactericidal activity of normal mouse peritoneal macrophages requires a viable, actively metabolizing cell. The killing mechanism is apparently dependent upon an intact respiratory electron chain since inhibition of activity is achieved by anaerobiosis, cyanide, antimycin A, or amytal. By way of contrast, inhibitors of oxidative phosphorylation, glycolysis, the Krebs citric acid cycle, and the phosphogluconate oxidative pathway are without influence on this antibacterial activity. Various dyes and electron acceptors with selected reduction-oxidation values also inhibit killing. Although the bactericidal substance was not identified, the data are consistent with the hypothesis that the primary agent is hydrogen peroxide.