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Infect Immun. 1971 June; 3(6): 784-792
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Stimulation of Enterotoxin B Production II. Synthetic Medium for Staphylococcal Growth and Enterotoxin B Production

Food Research Institute and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

A relatively large amount of enterotoxin B (125 µg/ml) was produced in a synthetic medium composed of amino acids, inorganic salts, and vitamins. This medium was prepared on the basis of the utilization of the various amino acids by Staphylococcus aureus strain S-6 throughout the growth cycle. Arginine appeared to be essential for enterotoxin production. The pattern of enterotoxin production in the synthetic media appeared to differ from that in complex media.