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Infect Immun. 1971 June; 3(6): 803-809
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Serological Properties of G and M Antibodies to the Somatic Antigen of Vibrio cholerae During the Course of Immunization of Rabbits

Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas 75235

ABSTRACT

Direct- and passive-agglutinating, complement-fixing, and bactericidal properties of G and M antibodies produced in rabbits inoculated with live Vibrio cholerae were determined at intervals over a period of 345 days. Although M antibody titers increased more rapidly than G during the initial stages of antibody production, the titers of G and M declined proportionally during a 3-month rest period and increased proportionally after a booster injection. The relative titers of M as determined in the four serological procedures remained fairly constant throughout the period of observation. In contrast, early G was less effective than late G in vibriocidal, complement-fixing, and passive-hemagglutinating activity. At no stage of immunization was the agglutinating ability of G affected by 2-mercaptoethanol, but its complement-dependent activity was markedly reduced, more so in early serum than in late. The heat lability of early G approached that of M, but G became more resistant to heat in later stages of immunization.