This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bekierkunst, A. Articles by Lederer, E. Search for Related Content PubMed PubMed Citation Articles by Bekierkunst, A. Articles by Lederer, E.

Immune Response to Sheep Red Blood Cells in Mice Pretreated with Mycobacterial Fractions

Department of Medical Bacteriology, Hebrew University-Hadassah Medical School, Jerusalem, Israel
Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France

ABSTRACT

Ten micrograms of trehalose-6,6' -dimycolate (cord factor) injected into the footpads of mice increased the antibody response to sheep red blood cells (SRBC) subsequently injected into the same sites. There is a relationship between the antibody response and the cellular reaction induced locally and in the draining lymph nodes by cord factor, as judged by a much weaker response when antigen is injected into the contralateral footpads. The time intervals between injection of cord factor and antigen were from 5 to 20 days. A similar increased antibody response to SRBC was evident after preliminary administration of Freund's complete adjuvant or living BCG bacilli into the footpads. There was no increased antibody response in mice pretreated with living BCG or Freund's adjuvant to SRBC injected into the contralateral footpads. Administration of wax D from human strains Peurois and Test was without any effect. Administration of SRBC emulsified in incomplete Freund's adjuvant containing 5 µg of cord factor induced a very strong antibody response in the mice as compared to that after injection of the same amount of antigen in incomplete Freund's adjuvant containing wax D or mycobacteria. The results are discussed.

This article has been cited by other articles:

• Fujita, Y., Naka, T., Doi, T., Yano, I. (2005). Direct molecular mass determination of trehalose monomycolate from 11 species of mycobacteria by MALDI-TOF mass spectrometry. Microbiology 151: 1443-1452 [Abstract] [Full Text]  
• Bekierkunst, A., Levij, I. S., Yarkoni, E., Vilkas, E., Lederer, E. (1971). Suppression of Urethan-Induced Lung Adenomas in Mice Treated with Trehalose-6,6-Dimycolate (Cord Factor) and Living Bacillus Calmette Guerin. Science 174: 1240-1242 [Abstract]