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Effect of Alkali-Treated Lipopolysaccharide on Erythrocyte Membrane Stability

I. inár¹ and J. W. Shands Jr.

^a Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville, Florida 32601

ABSTRACT

The interaction of various lipopolysaccharides (LPS) with sheep erythrocytes was studied. When subjected to mild alkaline hydrolysis, the affinity of LPS for the red cell surface was greatly increased, as others have reported. In addition, excessive quantities of alkali-treated LPS (but not parent or heated products) were found to cause hemolysis of red cells. Experiments indicated that the hemolysis was caused by the LPS particles themselves and not by liberated free fatty acids.

¹ Present address: Research Institute of Epidemiology and Microbiology, Sasinkova, Bratislava, Czechoslovakia.