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Purification of Pneumolysin

¹ Department of Pediatrics, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219
Department of Medicine, U. S. Public Health Service Hospital, University of Washington School of Medicine, Seattle, Washington 98114

ABSTRACT

A procedure for the purification of pneumolysin has been developed. Its use results in a 96-fold increase in specific activity from the original crude pneumococcal extract to the final purified product. The purified material is heat-labile and is inactivated by trypsin and cholesterol; inactivation by p-hydroxymercuribenzoate can be reversed by mercaptoethanol. Intravenous administration of the material to rabbits results in crenation, increased osmotic fragility, and intravascular lysis of the erythrocytes of the animal.

This article has been cited by other articles:

• Shoma, S., Tsuchiya, K., Kawamura, I., Nomura, T., Hara, H., Uchiyama, R., Daim, S., Mitsuyama, M. (2008). Critical Involvement of Pneumolysin in Production of Interleukin-1{alpha} and Caspase-1-Dependent Cytokines in Infection with Streptococcus pneumoniae In Vitro: a Novel Function of Pneumolysin in Caspase-1 Activation. Infect. Immun. 76: 1547-1557 [Abstract] [Full Text]