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In Vitro Attachment of Radioactive Endotoxins to Lysosomes

Centre de Physiologie et d'Immunologie Cellulaire, Hôpital St. Antoine, Centre National de la Recherche Scientifique, and Service de Chimiothérapie Expérimentale, Institut Pasteur, Paris, France

ABSTRACT

The experiments reported here demonstrate that under certain conditions endotoxin can interact with lysosomes in vitro. After incubation of large granular fraction with ⁵¹Cr-labeled antigen under the conditions required for acid hydrolytic activity, radioactivity was associated with the pellet after centrifugation. This effect can be inhibited by preincubation of the large granular fraction with unlabeled homologous or heterologous endotoxins. High resolution autoradiography showed that ¹⁴C-labeled endotoxin was predominantly attached to the lysosomes contained in the large granular fraction. The mechanism of this interaction and its relationship to the toxic effect of endotoxins on lysosomes are discussed.