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Infect Immun. 1971 November; 4(5): 575-580
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Cell-Free Passive Transfer of Delayed Hypersensitivity to Chemicals in Guinea Pigs

^a Department of Microbiology and Medical Technology, College of Liberal Arts, University of Arizona, Tucson, Arizona 85721

ABSTRACT

Delayed-type cutaneous reactivity to 2,4-dinitrochlorobenzene and 2,4-dinitrofluorobenzene in guinea pigs was transferred passively by fluids in which leukocytes from sensitive animals were incubated. Cells from peritoneal exudates, lymph nodes, and alveolar washings were employed. The cell-free transfer material was dialyzable, of small molecular size, and stable to 56 C for 30 min and –65 C for at least 9 weeks. It gave a ratio at 280 to 260 nm of 0.71. The relationships between temperature, pH, and cellular release of the transfer material were studied.

¹ Present address: Surgical Research Laboratory, Veterans Administration Hospital and Department of Microbiology, University of Oregon Medical School, Portland.

This article has been cited by other articles:

• Burger, D. R., Vetto, R. M., Malley, A. (1972). Transfer Factor from Guinea Pigs Sensitive to Dinitrochlorobenzene: Absence of Superantigen Properties. Science 175: 1473-1475 [Abstract]