This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Callahan, L. T. Articles by Richardson, S. H. Search for Related Content PubMed PubMed Citation Articles by Callahan, L. T., III Articles by Richardson, S. H.

Biochemistry of Vibrio cholerae Virulence II. Skin Permeability Factor/Cholera Enterotoxin Production in a Chemically Defined Medium

¹ Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103

ABSTRACT

A chemically defined medium capable of eliciting high titers of skin permeability factor (PF)/enterotoxin from three different strains of Vibrio cholerae has been developed. Toxin/antigen elaboration in synthetic and in complex media was monitored by a specific passive hemagglutination-inhibition test. A distinct temporal difference in the pattern of toxin/antigen elaboration was noted when the two types of media were compared. In complex media, PF activity and corresponding antigen release were coincident, whereas in the defined medium a biphasic pattern resulting in elaboration of nontoxic antigen during the second phase was seen. Possible reasons for the latter observation are discussed, and several experiments illustrating the unique utility of the defined medium are presented.

This article has been cited by other articles:

• Pratt, J. T., Tamayo, R., Tischler, A. D., Camilli, A. (2007). PilZ Domain Proteins Bind Cyclic Diguanylate and Regulate Diverse Processes in Vibrio cholerae. J. Biol. Chem. 282: 12860-12870 [Abstract] [Full Text]  
• Tischler, A. D., Camilli, A. (2005). Cyclic Diguanylate Regulates Vibrio cholerae Virulence Gene Expression. Infect. Immun. 73: 5873-5882 [Abstract] [Full Text]