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Infect Immun. 1983 May; 40(2): 665-669

Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa.

P A Sokol and D E Woods

ABSTRACT

Outer membranes prepared from Pseudomonas aeruginosa grown in low-iron medium bind three times as much [59Fe]pyochelin as do membranes from cells grown in high-iron medium. The deletion of pyochelin reduced 59Fe binding to background levels. Autoradiographic analysis of sodium dodecyl sulfate polyacrylamide gel electrophoretograms of outer membrane preparations previously incubated with [59Fe]pyochelin revealed that the iron-siderophore complex bound to a low-molecular-weight protein of approximately 14,000.

Infect Immun. 1983 May; 40(2): 665-669






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