This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Flamm, R K Articles by Thomashow, M F Search for Related Content PubMed PubMed Citation Articles by Flamm, R K Articles by Thomashow, M F

 Previous Article  |  Next Article 

Infect Immun. 1984 April; 44(1): 157-161

Introduction of pAM beta 1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids.

ABSTRACT

The broad host range antibiotic resistance plasmid pAM beta 1 was transferred from Streptococcus faecalis to 9 of 15 Listeria monocytogenes strains by conjugation. L. monocytogenes transconjugates could transfer the plasmid either among L. monocytogenes strains or back to S. faecalis. Transfer between the various strains occurred without any detectable plasmid DNA rearrangements. The pAM beta 1 replicon was stable in L. monocytogenes--it was retained without antibiotic selection when the bacteria were grown in culture media or passed in mice--and the presence of pAM beta 1 had no major effect on L. monocytogenes virulence. These data suggest that pAM beta 1 or its derivatives might serve as useful L. monocytogenes cloning vehicles. The data presented also demonstrate that pAM beta 1 is compatible with two different native L. monocytogenes plasmids and that Listeria species harbor native plasmids in addition to the 38.5-megadalton plasmid pRYC16 previously reported by Pérez-Díaz et al. (J. C. Pérez-Díaz, M. F. Vicente, and F. Banquero, Plasmid 8:112-118, 1982). Of 29 L. monocytogenes strains screened, 7 contained plasmid DNA. Four strains had similar if not identical plasmids that were 34 megadaltons in size, whereas three other strains contained either a 53-, 44-, or 32-megadalton plasmid; none of these plasmids has the same restriction patterns as pRYC16. DNA homology experiments indicate that the various plasmids are related and suggest that there may be a common set of sequences present in all of the plasmids examined.

This article has been cited by other articles:

• Raengpradub, S., Wiedmann, M., Boor, K. J. (2008). Comparative Analysis of the {sigma}B-Dependent Stress Responses in Listeria monocytogenes and Listeria innocua Strains Exposed to Selected Stress Conditions. Appl. Environ. Microbiol. 74: 158-171 [Abstract] [Full Text]  
• Chaturongakul, S., Boor, K. J. (2006). {sigma}B Activation under Environmental and Energy Stress Conditions in Listeria monocytogenes. Appl. Environ. Microbiol. 72: 5197-5203 [Abstract] [Full Text]  
• Tao, Y., Drabik, K. A., Waypa, T. S., Musch, M. W., Alverdy, J. C., Schneewind, O., Chang, E. B., Petrof, E. O. (2006). Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells. Am. J. Physiol. Cell Physiol. 290: C1018-C1030 [Abstract] [Full Text]  
• Xu, L., Li, H., Vuong, C., Vadyvaloo, V., Wang, J., Yao, Y., Otto, M., Gao, Q. (2006). Role of the luxS Quorum-Sensing System in Biofilm Formation and Virulence of Staphylococcus epidermidis. Infect. Immun. 74: 488-496 [Abstract] [Full Text]  
• Kim, H., Marquis, H., Boor, K. J. (2005). {sigma}B contributes to Listeria monocytogenes invasion by controlling expression of inlA and inlB. Microbiology 151: 3215-3222 [Abstract] [Full Text]  
• Palumbo, J. D., Kaneko, A., Nguyen, K. D., Gorski, L. (2005). Identification of Genes Induced in Listeria monocytogenes during Growth and Attachment to Cut Cabbage, Using Differential Display. Appl. Environ. Microbiol. 71: 5236-5243 [Abstract] [Full Text]  
• de Vin, F., Radstrom, P., Herman, L., De Vuyst, L. (2005). Molecular and Biochemical Analysis of the Galactose Phenotype of Dairy Streptococcus thermophilus Strains Reveals Four Different Fermentation Profiles. Appl. Environ. Microbiol. 71: 3659-3667 [Abstract] [Full Text]  
• Navarro-Llorens, J. M., Patrauchan, M. A., Stewart, G. R., Davies, J. E., Eltis, L. D., Mohn, W. W. (2005). Phenylacetate Catabolism in Rhodococcus sp. Strain RHA1: a Central Pathway for Degradation of Aromatic Compounds. J. Bacteriol. 187: 4497-4504 [Abstract] [Full Text]  
• Xue, J., Hunter, I., Steinmetz, T., Peters, A., Ray, B., Miller, K. W. (2005). Novel Activator of Mannose-Specific Phosphotransferase System Permease Expression in Listeria innocua, Identified by Screening for Pediocin AcH Resistance. Appl. Environ. Microbiol. 71: 1283-1290 [Abstract] [Full Text]  
• Sue, D., Fink, D., Wiedmann, M., Boor, K. J. (2004). {sigma}B-dependent gene induction and expression in Listeria monocytogenes during osmotic and acid stress conditions simulating the intestinal environment. Microbiology 150: 3843-3855 [Abstract] [Full Text]  
• Hedengren-Olcott, M., Olcott, M. C., Mooney, D. T., Ekengren, S., Geller, B. L., Taylor, B. J. (2004). Differential Activation of the NF-{kappa}B-like Factors Relish and Dif in Drosophila melanogaster by Fungi and Gram-positive Bacteria. J. Biol. Chem. 279: 21121-21127 [Abstract] [Full Text]  
• Wonderling, L. D., Wilkinson, B. J., Bayles, D. O. (2004). The htrA (degP) Gene of Listeria monocytogenes 10403S Is Essential for Optimal Growth under Stress Conditions. Appl. Environ. Microbiol. 70: 1935-1943 [Abstract] [Full Text]  
• Kazmierczak, M. J., Mithoe, S. C., Boor, K. J., Wiedmann, M. (2003). Listeria monocytogenes {sigma}B Regulates Stress Response and Virulence Functions. J. Bacteriol. 185: 5722-5734 [Abstract] [Full Text]  
• Wiens, G. D., Pascho, R., Winton, J. R. (2002). A Single Ala139-to-Glu Substitution in the Renibacterium salmoninarum Virulence-Associated Protein p57 Results in Antigenic Variation and Is Associated with Enhanced p57 Binding to Chinook Salmon Leukocytes. Appl. Environ. Microbiol. 68: 3969-3977 [Abstract] [Full Text]  
• Kohda, C., Kawamura, I., Baba, H., Nomura, T., Ito, Y., Kimoto, T., Watanabe, I., Mitsuyama, M. (2002). Dissociated Linkage of Cytokine-Inducing Activity and Cytotoxicity to Different Domains of Listeriolysin O from Listeria monocytogenes. Infect. Immun. 70: 1334-1341 [Abstract] [Full Text]  
• Taylor, C. M., Beresford, M., Epton, H. A. S., Sigee, D. C., Shama, G., Andrew, P. W., Roberts, I. S. (2002). Listeria monocytogenes relA and hpt Mutants Are Impaired in Surface-Attached Growth and Virulence. J. Bacteriol. 184: 621-628 [Abstract] [Full Text]  
• Wilson, R. L., Tvinnereim, A. R., Jones, B. D., Harty, J. T. (2001). Identification of Listeria monocytogenes In Vivo-Induced Genes by Fluorescence-Activated Cell Sorting. Infect. Immun. 69: 5016-5024 [Abstract] [Full Text]  
• Charpentier, E., Courvalin, P. (1999). Antibiotic Resistance in Listeria spp.. Antimicrob. Agents Chemother. 43: 2103-2108 [Full Text]  
• Ko, R., Smith, L. T. (1999). Identification of an ATP-Driven, Osmoregulated Glycine Betaine Transport System in Listeria monocytogenes. Appl. Environ. Microbiol. 65: 4040-4048 [Abstract] [Full Text]  
• Gilot, P., Gubina, M., Klun, N., Mehle, J., Tcherneva, E., Rijpens, N., Herman, L. (1999). Typing of Listeria monocytogenes Strains by Repetitive Element Sequence-Based PCR. J. Clin. Microbiol. 37: 103-109 [Abstract] [Full Text]  
• Wiedmann, M., Arvik, T. J., Hurley, R. J., Boor, K. J. (1998). General Stress Transcription Factor sigma B and Its Role in Acid Tolerance and Virulence of Listeria monocytogenes. J. Bacteriol. 180: 3650-3656 [Abstract] [Full Text]