This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uchiyama, T Articles by Yoshioka, M Search for Related Content PubMed PubMed Citation Articles by Uchiyama, T Articles by Yoshioka, M

 Previous Article  |  Next Article 

Infect Immun. 1984 August; 45(2): 367-371

Mechanism of lipopolysaccharide-induced immunosuppression: immunological activity of B cell subsets responding to T-dependent or T-independent antigens in lipopolysaccharide-preinjected mice.

ABSTRACT

Spleen cells from mice preinjected with high doses of bacterial lipopolysaccharide did not generate anti-trinitrophenyl (TNP) plaque-forming cells in vitro to the T-dependent antigen, TNP-sheep erythrocytes, but did generate fully plaque-forming cells to the T-independent antigens, TNP-Ficoll and TNP-Brucella abortus. The immunological activity of B cells from such lipopolysaccharide-preinjected mice was analyzed in the present study. T cell-depleted spleen cells from mice injected with 30 micrograms of lipopolysaccharide 3 days previously did not respond to combined stimulation with TNP-sheep erythrocytes and concanavalin A-induced T cell-replacing factor and had no suppressive activity on normal T cell-depleted spleen cells. Splenic B cells, which were separated from T cells and macrophages from mice injected with 30 micrograms of lipopolysaccharide 3 days previously, responded only partially (about 25% of the control response) to combined stimulation with TNP-sheep erythrocytes and concanavalin A-induced cell-replacing factor in the presence of normal macrophages, but responded fully to TNP-B. abortus, regardless of the presence of normal macrophages. These results indicate that B cells responding to the T-dependent antigens are rendered unresponsive to antigenic stimulation in mice preinjected with lipopolysaccharide, whereas B cells responding to the T-independent antigens are kept intact.

This article has been cited by other articles:

• Forestier, C., Deleuil, F., Lapaque, N., Moreno, E., Gorvel, J.-P. (2000). Brucella abortus Lipopolysaccharide in Murine Peritoneal Macrophages Acts as a Down-Regulator of T Cell Activation. J. Immunol. 165: 5202-5210 [Abstract] [Full Text]