This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frank, D W
Right arrow Articles by Parker, C D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frank, D W
Right arrow Articles by Parker, C D

 Previous Article  |  Next Article 

Infect Immun. 1984 October; 46(1): 195-201

Interaction of monoclonal antibodies with pertussis toxin and its subunits.

D W Frank and C D Parker

ABSTRACT

The binding of two monoclonal antibodies to crude and pure preparations of pertussis toxin was examined. Antibody P11B10 reacted with an epitope present on the S2 subunit of pertussis toxin by immunoblot techniques. Radioimmunoprecipitation analysis and immunoaffinity chromatography with P11B10 indicated that subunits S2 and S3 were closely associated. Antibody P7B10 was unreactive in immunoblots and radioimmunoprecipitation but was able to bind and retain toxin subunits during affinity chromatography. The P7B10 epitope may thus be labile to detergent treatment or radioiodination; or the epitope may form as a result of subunit association. Neither antibody alone nor both in combination neutralized the histamine-sensitizing activity of pertussis toxin in passive-transfer experiments. However, toxin isolated by immunoaffinity chromatography with either monoclonal antibody was able to sensitize mice to histamine challenge.

Infect Immun. 1984 October; 46(1): 195-201




This article has been cited by other articles:

  • Burns, D. L., Fiddner, S., Cheung, A. M., Verma, A. (2004). Analysis of Subassemblies of Pertussis Toxin Subunits In Vivo and Their Interaction with the Ptl Transport Apparatus. Infect. Immun. 72: 5365-5372 [Abstract] [Full Text]  
  • Cheung, A. M., Farizo, K. M., Burns, D. L. (2004). Analysis of Relative Levels of Production of Pertussis Toxin Subunits and Ptl Proteins in Bordetella pertussis. Infect. Immun. 72: 2057-2066 [Abstract] [Full Text]  
  • Stenson, T. H., Weiss, A. A. (2002). DsbA and DsbC Are Required for Secretion of Pertussis Toxin by Bordetella pertussis. Infect. Immun. 70: 2297-2303 [Abstract] [Full Text]  
  • Farizo, K. M., Huang, T., Burns, D. L. (2000). Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from Bordetella pertussis. Infect. Immun. 68: 4049-4054 [Abstract] [Full Text]  
  • Hausman, S. Z., Burns, D. L. (2000). Use of Pertussis Toxin Encoded by ptx Genes from Bordetella bronchiseptica To Model the Effects of Antigenic Drift of Pertussis Toxin on Antibody Neutralization. Infect. Immun. 68: 3763-3767 [Abstract] [Full Text]  
  • Kang, H. Y., Armstrong, S. K. (1998). Transcriptional Analysis of the Bordetella Alcaligin Siderophore Biosynthesis Operon. J. Bacteriol. 180: 855-861 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»