This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hentges, D J Articles by Que, J U Search for Related Content PubMed PubMed Citation Articles by Hentges, D J Articles by Que, J U

 Previous Article  |  Next Article 

Infect Immun. 1985 January; 47(1): 118-122

Protective role of intestinal flora against infection with Pseudomonas aeruginosa in mice: influence of antibiotics on colonization resistance.

ABSTRACT

Swiss white mice were given ampicillin, clindamycin, kanamycin, metronidazole, or streptomycin in drinking water for a period of 3 weeks. One week after the initiation of antibiotic administration, the treated mice and untreated control mice were challenged orally with approximately 10(8) viable, streptomycin-resistant (SR) Pseudomonas aeruginosa isolates. All five of the antibiotics decreased the resistance of the mice to intestinal colonization with SR P. aeruginosa, as reflected by an increased fecal carriage of the organism and an increase in population levels of SR P. aeruginosa in feces as compared with untreated controls. Metronidazole was least effective in this regard. The antibiotics lowered the dose of SR P. aeruginosa that resulted in implantation in 50% of the mice ID50 to various degrees. Administration of streptomycin, the most effective antibiotic, caused a 10,000-fold decrease in ID50 as compared with untreated controls. Oral inoculation of approximately 10(8) organisms of SR P. aeruginosa resulted in translocation of the organism to the mesenteric lymph nodes, spleens, or livers of 13 or 17 streptomycin-treated mice, 1 of 20 clindamycin-treated mice, and 1 of 14 metronidazole-treated mice. Translocation was not observed, however, in ampicillin- or kanamycin-treated animals. Antibiotic activity was detected in the cecal contents of streptomycin-, kanamycin, and clindamycin-treated mice but not in the cecal contents of ampicillin- or metronidazole-treated animals.

This article has been cited by other articles:

• de Jong, P., Vissers, M. M. M., van der Meer, R., Bovee-Oudenhoven, I. M. J. (2007). In Silico Model as a Tool for Interpretation of Intestinal Infection Studies. Appl. Environ. Microbiol. 73: 508-515 [Abstract] [Full Text]  
• Sarma-Rupavtarm, R. B., Ge, Z., Schauer, D. B., Fox, J. G., Polz, M. F. (2004). Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract. Appl. Environ. Microbiol. 70: 2791-2800 [Abstract] [Full Text]  
• MIYAZAKI, S., FUJIKAWA, T., KOBAYASHI, I., MATSUMOTO, T., TATEDA, K., YAMAGUCHI, K. (2001). Development of systemic bacteraemia after oral inoculation of vancomycin-resistant enterococci in mice. J Med Microbiol 50: 695-701 [Abstract] [Full Text]  
• Sandler, R. H., Finegold, S. M., Bolte, E. R., Buchanan, C. P., Maxwell, A. P., Vaisanen, M.-L., Nelson, M. N., Wexler, H. M. (2000). Short-Term Benefit From Oral Vancomycin Treatment of Regressive-Onset Autism. J Child Neurol 15: 429-435 [Abstract]  
• Dewhirst, F. E., Chien, C.-C., Paster, B. J., Ericson, R. L., Orcutt, R. P., Schauer, D. B., Fox, J. G. (1999). Phylogeny of the Defined Murine Microbiota: Altered Schaedler Flora. Appl. Environ. Microbiol. 65: 3287-3292 [Abstract] [Full Text]