This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamada, T
Right arrow Articles by Abbe, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamada, T
Right arrow Articles by Abbe, K

 Previous Article  |  Next Article 

Infect Immun. 1985 January; 47(1): 129-134

Effects of oxygen on pyruvate formate-lyase in situ and sugar metabolism of Streptococcus mutans and Streptococcus sanguis.

T Yamada, S Takahashi-Abbe and K Abbe

ABSTRACT

The strictly anaerobic metabolism of sugar in strains of Streptococcus mutans and Streptococcus sanguis was studied because deep layers of dental plaque are strictly anaerobic. Galactose-grown cells of these streptococcal strains had higher pyruvate formate-lyase activity than did glucose-grown cells. Among these strains, two strains of S. mutans had a significantly higher pyruvate formate-lyase activity than did the others. This enzyme is extremely sensitive to oxygen, and even in situ the enzyme was inactivated by exposure of the cells to air. Lactate was less than 50% of the total end product of the strictly anaerobic incubation of the galactose-grown cells of S. mutans with excess glucose, and a significant amount of formate, acetate, and ethanol was produced through the catalysis of pyruvate formate-lyase. But the cells exclusively produced lactate when exposed to air for 2 min before the anaerobic incubation. The metabolism of sorbitol by S. mutans was seriously impaired by the exposure of the cells to oxygen, and the metabolic rate was reduced to less than 1/20 of that found under strictly anaerobic conditions because of the inactivation of pyruvate formate-lyase. S. sanguis produced a smaller amount of the volatile products from glucose than did S. mutans because of the low level of pyruvate formate-lyase. However, the pyruvate formate-lyase in situ in S. sanguis was less sensitive to oxygen than was that in S. mutans. Because of this low sensitivity, S. sanguis metabolized glucose more rapidly under aerobic conditions, whereas the rates of the aerobic and anaerobic metabolism of glucose by S. mutans were similar, which suggests that S. mutans rather than S. sanguis can sustain the rapid sugar metabolism in the deep layers of dental plaque.

Infect Immun. 1985 January; 47(1): 129-134




This article has been cited by other articles:

  • Yesilkaya, H., Spissu, F., Carvalho, S. M., Terra, V. S., Homer, K. A., Benisty, R., Porat, N., Neves, A. R., Andrew, P. W. (2009). Pyruvate Formate Lyase Is Required for Pneumococcal Fermentative Metabolism and Virulence. Infect. Immun. 77: 5418-5427 [Abstract] [Full Text]  
  • Bekker, M., de Vries, S., Ter Beek, A., Hellingwerf, K. J., de Mattos, M. J. T. (2009). Respiration of Escherichia coli Can Be Fully Uncoupled via the Nonelectrogenic Terminal Cytochrome bd-II Oxidase. J. Bacteriol. 191: 5510-5517 [Abstract] [Full Text]  
  • Beckloff, N., Laube, D., Castro, T., Furgang, D., Park, S., Perlin, D., Clements, D., Tang, H., Scott, R. W., Tew, G. N., Diamond, G. (2007). Activity of an Antimicrobial Peptide Mimetic against Planktonic and Biofilm Cultures of Oral Pathogens. Antimicrob. Agents Chemother. 51: 4125-4132 [Abstract] [Full Text]  
  • Hasona, A., Kim, Y., Healy, F. G., Ingram, L. O., Shanmugam, K. T. (2004). Pyruvate Formate Lyase and Acetate Kinase Are Essential for Anaerobic Growth of Escherichia coli on Xylose. J. Bacteriol. 186: 7593-7600 [Abstract] [Full Text]  
  • Kohler, C., von Eiff, C., Peters, G., Proctor, R. A., Hecker, M., Engelmann, S. (2003). Physiological Characterization of a Heme-Deficient Mutant of Staphylococcus aureus by a Proteomic Approach. J. Bacteriol. 185: 6928-6937 [Abstract] [Full Text]  
  • Jensen, N. B. S., Melchiorsen, C. R., Jokumsen, K. V., Villadsen, J. (2001). Metabolic Behavior of Lactococcus lactis MG1363 in Microaerobic Continuous Cultivation at a Low Dilution Rate. Appl. Environ. Microbiol. 67: 2677-2682 [Abstract] [Full Text]  
  • Melchiorsen, C. R., Jokumsen, K. V., Villadsen, J., Johnsen, M. G., Israelsen, H., Arnau, J. (2000). Synthesis and Posttranslational Regulation of Pyruvate Formate-Lyase in Lactococcus lactis. J. Bacteriol. 182: 4783-4788 [Abstract] [Full Text]  
  • Yamamoto, Y., Sato, Y., Takahashi-Abbe, S., Takahashi, N., Kizaki, H. (2000). Characterization of the Streptococcus mutans Pyruvate Formate-Lyase (PFL)-Activating Enzyme Gene by Complementary Reconstitution of the In Vitro PFL-Reactivating System. Infect. Immun. 68: 4773-4777 [Abstract] [Full Text]  
  • Bowden, G. H.W., Hamilton, I. R. (1998). Survival of Oral Bacteria. CROBM 9: 54-85 [Abstract] [Full Text]  
  • Guha-Chowdhury, N., Iwami, Y., Yamada, T., Pearce, E.I.F. (1995). The Effect of Fluorhydroxyapatite-derived Fluoride on Acid Production by Streptococci. JDR 74: 1618-1624 [Abstract]  
  • Hata, S., Iwami, Y., Kamiyama, K., Yamada, T. (1990). Biochemical Mechanisms of Enhanced Inhibition of Fluoride on the Anaerobic Sugar Metabolism by Streptococcus sanguis. JDR 69: 1244-1247 [Abstract]  
  • Iwami, Y., Hata, S., Takahashi, N., Yamada, T. (1989). Difference in Amounts between Titratable Acid and Total Carboxylic Acids Produced by Oral Streptococci during Sugar Metabolism. JDR 68: 16-19 [Abstract]  
  • Abhyankar, S., Sandham, H.J., Chan, K.H. (1985). Serotype c Streptococcus mutans Mutatable to Lactate Dehydrogenase Deficiency. JDR 64: 1267-1271 [Abstract]  
  • Svensater, G., Takahashi-Abbe, S., Abbe, K., Birkhed, D., Yamada, T., Edwardsson, S. (1985). Anaerobic and Aerobic Metabolism of Sorbitol in Streptococcus sanguis and Streptococcus mitior. JDR 64: 1286-1289 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»