This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oda, M Articles by Manclark, C R Search for Related Content PubMed PubMed Citation Articles by Oda, M Articles by Manclark, C R

 Previous Article  |  Next Article 

Infect Immun. 1985 February; 47(2): 441-445

Antibodies to Bordetella pertussis in human colostrum and their protective activity against aerosol infection of mice.

ABSTRACT

Colostrum samples from Indonesian mothers were assayed for antibodies which agglutinate Bordetella pertussis and for antibodies to the filamentous hemagglutinin and the lymphocytosis-promoting factor of B. pertussis. Agglutinins were assayed by a microtiter method, and 36 of 58 samples tested (62%) had titers above 1:10 (range, less than 1:10 to 1:160). An enzyme-linked immunosorbent assay detected anti-filamentous hemagglutinin in 39 of 60 samples (65%) and anti-lymphocytosis-promoting factor in 26 of 60 samples assayed (43%). A total of 52 samples (87%) were positive for at least one of these antibodies. Pooled colostrum samples were separated by affinity chromatography into fractions enriched secretory immunoglobulin A (sIgA) or IgG and examined for their ability to passively protect suckling mice from aerosol challenge with B. pertussis. Samples (160 micrograms of protein) were given intraperitoneally 90 min before challenge. Death, rate of gain in body weight, and leukocytosis were used as indicators of illness. Colostrum containing anti-lymphocytosis-promoting factor or agglutinins was protective, whereas colostrum lacking these but containing anti-filamentous hemagglutinin gave little protection. The sIgA-enriched and IgG-enriched fractions appeared to be equal in their ability to protect against respiratory challenge with B. pertussis.

This article has been cited by other articles:

• Elahi, S., Brownlie, R., Korzeniowski, J., Buchanan, R., O'Connor, B., Peppler, M. S., Halperin, S. A., Lee, S. F., Babiuk, L. A., Gerdts, V. (2005). Infection of Newborn Piglets with Bordetella pertussis: a New Model for Pertussis. Infect. Immun. 73: 3636-3645 [Abstract] [Full Text]  
• Mills, K. H. G., Ryan, M., Ryan, E., Mahon, B. P. (1998). A Murine Model in Which Protection Correlates with Pertussis Vaccine Efficacy in Children Reveals Complementary Roles for Humoral and Cell-Mediated Immunity in Protection against Bordetella pertussis. Infect. Immun. 66: 594-602 [Abstract] [Full Text]