This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Knutton, S Articles by McNeish, A S Search for Related Content PubMed PubMed Citation Articles by Knutton, S Articles by McNeish, A S

 Previous Article  |  Next Article 

Infect Immun. 1985 June; 48(3): 824-831

Adhesion of enterotoxigenic Escherichia coli to human small intestinal enterocytes.

ABSTRACT

An improved enterocyte adhesion assay has been used to examine a collection of 44 strains of enterotoxigenic Escherichia coli (ETEC) for their ability to adhere to the brush border of isolated human duodenal enterocytes. Fourteen strains showed good adhesion; in each case the ability to adhere correlated with the production of colonization factor antigen I or II (CFA/I or CFA/II) fimbriae. CFA/II-positive producing coli surface antigens 1 and 3 (CS1 and CS3), coli surface antigens 2 and 3 (CS2 and CS3), and only coli surface antigen 3 (CS3) each showed good adhesion. CS3-mediated brush border attachment of CFA/II-positive ETEC was demonstrated by electron microscopy with monospecific antibody and an immunogold labeling technique. One CFA/I-positive ETEC strain was nonadherent in the assay, as were ETEC producing type 1 somatic fimbriae. Five animal ETEC strains producing K88, K99, F41, and 987P fimbriae were slightly more adhesive than control strains, but adhesion was significantly less than that of CFA-positive ETEC. Twenty five human ETEC strains that lacked CFA/I and CFA/II were nonadherent, suggesting either that the surface antigens responsible for adhesion to human intestinal mucosa in these strains were not being produced or that mucosal receptors for these strains are present in regions of the small intestine other than the duodenum.

This article has been cited by other articles:

• Nuccio, S.-P., Baumler, A. J. (2007). Evolution of the Chaperone/Usher Assembly Pathway: Fimbrial Classification Goes Greek. Microbiol. Mol. Biol. Rev. 71: 551-575 [Abstract] [Full Text]  
• Li, Y.-F., Poole, S., Rasulova, F., McVeigh, A. L., Savarino, S. J., Xia, D. (2007). A Receptor-binding Site as Revealed by the Crystal Structure of CfaE, the Colonization Factor Antigen I Fimbrial Adhesin of Enterotoxigenic Escherichia coli. J. Biol. Chem. 282: 23970-23980 [Abstract] [Full Text]  
• Ludi, S., Frey, J., Favre, D., Stoffel, M. H. (2006). Assessing the Expression of Enterotoxigenic Escherichia coli-specific Surface Antigens in Recombinant Strains by Transmission Electron Microscopy and Immunolabeling. J. Histochem. Cytochem. 54: 473-477 [Abstract] [Full Text]  
• Roselli, M., Finamore, A., Garaguso, I., Britti, M. S., Mengheri, E. (2003). Zinc Oxide Protects Cultured Enterocytes from the Damage Induced by Escherichia coli. J. Nutr. 133: 4077-4082 [Abstract] [Full Text]  
• Kirov, S. M., O'Donovan, L. A., Sanderson, K. (1999). Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species. Infect. Immun. 67: 5447-5454 [Abstract] [Full Text]