This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shaio, M F Articles by Rowland, H Search for Related Content PubMed PubMed Citation Articles by Shaio, M F Articles by Rowland, H

 Previous Article  |  Next Article 

Infect Immun. 1985 September; 49(3): 647-653

Bactericidal and opsonizing effects of normal serum on mutant strains of Salmonella typhimurium.

ABSTRACT

The bactericidal and opsonizing effects of normal human serum on six strains of Salmonella typhimurium LT-2 having different lipopolysaccharide (LPS) composition were demonstrated through five indices. Complement activity in the presence of antibody was important for the opsonization of all six strains and for the bactericidal effect on rough mutants. Complement activity, either in the presence or absence of antibody, was involved in the ingestion strains of SL 901 (SR) and SL 1032 (Rd1) by human neutrophils. Strain SH 5014 (Rb2) was avidly ingested by neutrophils and totally dependent on complement activity in the presence of antibody. The ingestion of strain SH 2201 (S) was also mediated exclusively by complement activity in the presence of antibody but not as efficiently as were rough mutants. Antibody, as demonstrated by quantitative fluorescence, enhanced the complement activity on the ingestion of the S, SR, and Rb2 strains by neutrophils. The intracellular killing of six strains was enhanced significantly by complement activity in the presence of antibody. The overall survival in the presence of serum and neutrophils decreased as the LPS became shorter. Complement activity in the presence of antibody enhanced extracellular killing only for strains SL 901 (SR) and his 515 (Ra). It was shown that there was no difference between SR and Ra strains in all five indices, suggesting that the one additional O-antigen side chain does not make the SR strain more resistant than the Ra strain. Although resistance by S. typhimurium to host defense mechanisms increases as the LPS chain length increases, the specific LPS structure appears to be of greater importance, especially with respect to opsonization.

This article has been cited by other articles:

• Sebkova, A., Karasova, D., Crhanova, M., Budinska, E., Rychlik, I. (2008). aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity. J. Bacteriol. 190: 3155-3160 [Abstract] [Full Text]  
• van Bruggen, R., Zweers, D., van Diepen, A., van Dissel, J. T., Roos, D., Verhoeven, A. J., Kuijpers, T. W. (2007). Complement Receptor 3 and Toll-Like Receptor 4 Act Sequentially in Uptake and Intracellular Killing of Unopsonized Salmonella enterica Serovar Typhimurium by Human Neutrophils. Infect. Immun. 75: 2655-2660 [Abstract] [Full Text]  
• Nagy, G., Danino, V., Dobrindt, U., Pallen, M., Chaudhuri, R., Emody, L., Hinton, J. C., Hacker, J. (2006). Down-Regulation of Key Virulence Factors Makes the Salmonella enterica Serovar Typhimurium rfaH Mutant a Promising Live-Attenuated Vaccine Candidate.. Infect. Immun. 74: 5914-5925 [Abstract] [Full Text]  
• Jones, M. A., Marston, K. L., Woodall, C. A., Maskell, D. J., Linton, D., Karlyshev, A. V., Dorrell, N., Wren, B. W., Barrow, P. A. (2004). Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract. Infect. Immun. 72: 3769-3776 [Abstract] [Full Text]  
• Carlson, S. A., Jones, B. D. (1998). Inhibition of Salmonella typhimurium Invasion by Host Cell Expression of Secreted Bacterial Invasion Proteins. Infect. Immun. 66: 5295-5300 [Abstract] [Full Text]