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Infect Immun. 1972 May; 5(5): 775-782
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Contribution of Humoral and Cellular Factors to the Resistance to Experimental Infection by Pseudomonas aeruginosa in Mice II. Opsonic, Agglutinative, and Protective Capacities of Immunoglobulin G Anti-Pseudomonas Antibodies

¹ Department of Microbiology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45219

ABSTRACT

The opsonic, agglutinative, and mouse protective capacities of anti-Pseudomonas antibodies in immune and normal human immunoglobulin G (IgG) preparations were investigated. Opsonic activities of the immune IgG preparations correlated well with their protective activities. Antibodies present in normal IgG showed a substantial agglutinative activity but were poorly protective. Anti-Pseudomonas antibodies of both normal and immune IgG preparations were directed against the same serotype antigen as demonstrated by absorption experiments. Immune anti-Pseudomonas IgG antibodies fixed complement very efficiently as demonstrated by opsonophagocytic and hemolytic tests. Natural anti-Pseudomonas IgG antibodies fixed complement very poorly although they promoted phagocytic killing of bacteria only in the presence of heat-labile serum factors. It was concluded that, although agglutination can be used for qualitative measurement of antibacterial antibodies, it fails to measure their functional capacities.