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Specific and Nonspecific Staining in Detecting Staphylococcal Alpha Toxin on Erythrocytes by Immunofluorescence

Curriculum of Environmental Interactions, California College of Medicine, University of California, Irvine, California 92664

ABSTRACT

Specifically stained staphylococcal alpha toxin particles were detected on unstained and nonspecifically stained ghost membranes when rabbit erythrocytes were exposed to alpha toxin and stained with fluorescein isothiocyanate-labeled specific antitoxin. Fading of the nonspecific staining was observed and was attributed to the degradation of the membranes by alpha toxin.