This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klotz, S A Articles by Zajic, J E Search for Related Content PubMed PubMed Citation Articles by Klotz, S A Articles by Zajic, J E

 Previous Article  |  Next Article 

Infect Immun. 1985 October; 50(1): 97-101

Factors governing adherence of Candida species to plastic surfaces.

ABSTRACT

The ability of Candida albicans and Candida spp. to adhere to inert polymeric surfaces may allow these organisms direct ingress into the human host. Biophysical characterization of this adherence shows that the forces responsible for such adherence are attractive London-van der Waals forces (or hydrophobic forces) and electrostatic forces. The hydrophobic affinity of yeasts was determined by (i) a water-hydrocarbon two-phase assay and by (ii) measurement of the contact angle (theta) of a liquid droplet on a monolayer of yeast cells. The hydrophobicity of the yeasts correlated with the tendency of yeasts to adhere to polystyrene and was reduced in the presence of Tween 20. The adherence of yeasts to polymers of increasing hydrophobicity (determined by the contact angle method) was directly proportional to theta. Yeast surface charges were altered by selectively blocking amino and carboxyl groups. The more positively charged yeasts adhered in greater numbers. Increasing the molarity of NaCl increased yeast adherence. These forces probably contribute to the negative cooperativity (determined by Scatchard and Hill plot) that characterizes the adherence of yeasts to polymers.

This article has been cited by other articles:

• Goldenberg, O., Herrmann, S., Adam, T., Marjoram, G., Hong, G., Gobel, U. B., Graf, B. (2005). Use of Denaturing High-Performance Liquid Chromatography for Rapid Detection and Identification of Seven Candida Species. J. Clin. Microbiol. 43: 5912-5915 [Abstract] [Full Text]  
• Cao, Y.-Y., Cao, Y.-B., Xu, Z., Ying, K., Li, Y., Xie, Y., Zhu, Z.-Y., Chen, W.-S., Jiang, Y.-Y. (2005). cDNA Microarray Analysis of Differential Gene Expression in Candida albicans Biofilm Exposed to Farnesol. Antimicrob. Agents Chemother. 49: 584-589 [Abstract] [Full Text]  
• Li, X., Yan, Z., Xu, J. (2003). Quantitative variation of biofilms among strains in natural populations of Candida albicans. Microbiology 149: 353-362 [Abstract] [Full Text]  
• Gallardo-Moreno, A. M., Gonzalez-Martin, M. L., Perez-Giraldo, C., Garduno, E., Bruque, J. M., Gomez-Garcia, A. C. (2002). Thermodynamic Analysis of Growth Temperature Dependence in the Adhesion of Candida parapsilosis to Polystyrene. Appl. Environ. Microbiol. 68: 2610-2613 [Abstract] [Full Text]  
• Ashbee, H. R., Evans, E. G. V. (2002). Immunology of Diseases Associated with Malassezia Species. Clin. Microbiol. Rev. 15: 21-57 [Abstract] [Full Text]  
• de Repentigny, L., Aumont, F., Bernard, K., Belhumeur, P. (2000). Characterization of Binding of Candida albicans to Small Intestinal Mucin and Its Role in Adherence to Mucosal Epithelial Cells. Infect. Immun. 68: 3172-3179 [Abstract] [Full Text]  
• Gaur, N. K., Klotz, S. A., Henderson, R. L. (1999). Overexpression of the Candida albicans ALA1 Gene in Saccharomyces cerevisiae Results in Aggregation following Attachment of Yeast Cells to Extracellular Matrix Proteins, Adherence Properties Similar to Those of Candida albicans. Infect. Immun. 67: 6040-6047 [Abstract] [Full Text]  
• Webb, J. S., Van der Mei, H. C., Nixon, M., Eastwood, I. M., Greenhalgh, M., Read, S. J., Robson, G. D., Handley, P. S. (1999). Plasticizers Increase Adhesion of the Deteriogenic Fungus Aureobasidium pullulans to Polyvinyl Chloride. Appl. Environ. Microbiol. 65: 3575-3581 [Abstract] [Full Text]  
• Buck, J. W., Andrews, J. H. (1999). Localized, Positive Charge Mediates Adhesion of Rhodosporidium toruloides to Barley Leaves and Polystyrene. Appl. Environ. Microbiol. 65: 2179-2183 [Abstract] [Full Text]  
• Fidel, P. L. Jr., Vazquez, J. A., Sobel, J. D. (1999). Candida glabrata: Review of Epidemiology, Pathogenesis, and Clinical Disease with Comparison to C. albicans. Clin. Microbiol. Rev. 12: 80-96 [Abstract] [Full Text]  
• Chaffin, W. L., Lopez-Ribot, J. L., Casanova, M., Gozalbo, D., Martinez, J. P. (1998). Cell Wall and Secreted Proteins of Candida albicans: Identification, Function, and Expression. Microbiol. Mol. Biol. Rev. 62: 130-180 [Abstract] [Full Text]  
• Robert, R., Faure, O., Carloti, A., Lebeau, B., Bernard, C., Marot-Leblond, A., Grillot, R., Senet, J.-M. (1998). A Monoclonal Antibody Specific to Surface Antigen on Candida krusei. CVI 5: 121-124 [Abstract] [Full Text]  
• Neu, T. R., Marshall, K. C. (1990). Bacterial Polymers: Physicochemical Aspects of Their Interactions at Interfaces. J Biomater Appl 5: 107-133 [Abstract]