This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Newsome, A L Articles by Arnold, R R Search for Related Content PubMed PubMed Citation Articles by Newsome, A L Articles by Arnold, R R

 Previous Article  |  Next Article 

Infect Immun. 1985 November; 50(2): 449-452

Interactions between Naegleria fowleri and Legionella pneumophila.

ABSTRACT

Using electron microscopy we documented some of the intracellular events that occur in Naegleria fowleri suspended in Page amoeba saline after ingestion of Legionella pneumophila. Photomicrographs showed intracellular vacuoles containing bacteria in the process of binary fission that was accompanied by alignment of mitochondria and ribosome-like structures along the vacuole membrane. Although these intracellular events are remarkably similar to that seen in Legionella replication within human monocytes, we could not demonstrate an increase in the number of bacteria by CFU or dark-field microscopy. However, when the Naegleria cells were allowed to ingest Legionella cells while suspended in amoeba culture medium, the number of bacteria increased, and this was contingent upon the presence of viable amoebae.

This article has been cited by other articles:

• Molmeret, M., Horn, M., Wagner, M., Santic, M., Abu Kwaik, Y. (2005). Amoebae as Training Grounds for Intracellular Bacterial Pathogens. Appl. Environ. Microbiol. 71: 20-28 [Full Text]  
• Cirillo, S. L. G., Yan, L., Littman, M., Samrakandi, M. M., Cirillo, J. D. (2002). Role of the Legionella pneumophila rtxA gene in amoebae. Microbiology 148: 1667-1677 [Abstract] [Full Text]  
• Segal, G., Shuman, H. A. (1999). Legionella pneumophila Utilizes the Same Genes To Multiply within Acanthamoeba castellanii and Human Macrophages. Infect. Immun. 67: 2117-2124 [Abstract] [Full Text]  
• Fabbi, M., Pastoris, M. C., Scanziani, E., Magnino, S., Di Matteo, L. (1998). Epidemiological and Environmental Investigations of Legionella pneumophila Infection in Cattle and Case Report of Fatal Pneumonia in a Calf. J. Clin. Microbiol. 36: 1942-1947 [Abstract] [Full Text]  
• Newsome, A. L., Scott, T. M., Benson, R. F., Fields, B. S. (1998). Isolation of an Amoeba Naturally Harboring a Distinctive Legionella Species. Appl. Environ. Microbiol. 64: 1688-1693 [Abstract] [Full Text]  
• Rohr, U., Weber, S., Michel, R., Selenka, F., Wilhelm, M. (1998). Comparison of Free-Living Amoebae in Hot Water Systems of Hospitals with Isolates from Moist Sanitary Areas by Identifying Genera and Determining Temperature Tolerance. Appl. Environ. Microbiol. 64: 1822-1824 [Abstract] [Full Text]