This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tellefson, L M Articles by Germaine, G R Search for Related Content PubMed PubMed Citation Articles by Tellefson, L M Articles by Germaine, G R

Adherence of Streptococcus sanguis to hydroxyapatite coated with lysozyme and lysozyme-supplemented saliva.

ABSTRACT

The adherence of [3H]thymidine-labeled Streptococcus sanguis strains to bare hydroxyapatite and to hydroxyapatite coated with a range of concentrations of lysozyme, poly-L-lysine, poly-L-glutamic acid, whole saliva supernatant, and combinations of some of the above was studied. Adherence of several strains of S. sanguis to bare hydroxyapatite and saliva-coated hydroxyapatite was compared. Saliva present as a pellicle on the hydroxyapatite inhibited adherence of some strains (903, M-5, 73X11) and stimulated that of others (S35, B-4, 66X49). Strains 903 and S35 were chosen for further study. Adherence of both strains was stimulated up to fivefold by the presence of adsorbed lysozyme or poly-L-lysine on the hydroxyapatite, whereas poly-L-glutamic acid inhibited adherence (80 to 95%). Adherence of strain S35 to hydroxyapatite coated with combinations of saliva and (i) lysozyme, (ii) poly-L-lysine, or (iii) poly-L-glutamic acid was unaffected compared with adherence to hydroxyapatite coated with saliva alone. In contrast, adherence of strain 903 to hydroxyapatite coated with combinations of saliva and either lysozyme or poly-L-lysine was inhibited up to ca. 90% compared with hydroxyapatite coated with saliva alone. Strain 903 was also unaffected by combinations of poly-L-glutamic acid and saliva on the hydroxyapatite. Adherent cells of both strains were completely (greater than 90%) eluted with high-ionic-strength buffer from either bare hydroxyapatite or hydroxyapatite coated with lysozyme alone. Adherent cells of strain S35 were only poorly eluted (25%) from hydroxyapatite coated with either saliva alone or saliva and lysozyme. Strain 903 elution from hydroxyapatite coated with either saliva alone or saliva and lysozyme was essentially complete. These observations were taken to indicate that the two test strains adhered to saliva-coated hydroxyapatite by different mechanisms. Protein-coated hydroxyapatite was shown not to be saturated under the conditions described here. Examination by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the variously supplemented salivary pellicles formed on the hydroxyapatite demonstrated that major changes in salivary protein composition did not occur when lysozyme, poly-L-lysine, or poly-L-glutamic acid was used to supplement saliva. Lysozyme-dependent aggregation of strain 903 was shown not to occur under the conditions of our experiments. We suggest that the basis for stimulation of adherence to hydroxyapatite coated only with lysozyme is an increase in the cationic surface area available for electrostatic adherence of the microorganisms.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

• Nobbs, A. H., Zhang, Y., Khammanivong, A., Herzberg, M. C. (2007). Streptococcus gordonii Hsa Environmentally Constrains Competitive Binding by Streptococcus sanguinis to Saliva-Coated Hydroxyapatite. J. Bacteriol. 189: 3106-3114 [Abstract] [Full Text]  
• Zhang, Y., Lei, Y., Nobbs, A., Khammanivong, A., Herzberg, M. C. (2005). Inactivation of Streptococcus gordonii SspAB Alters Expression of Multiple Adhesin Genes. Infect. Immun. 73: 3351-3357 [Abstract] [Full Text]  
• Zhang, Y., Lei, Y., Khammanivong, A., Herzberg, M. C. (2004). Identification of a Novel Two-Component System in Streptococcus gordonii V288 Involved in Biofilm Formation. Infect. Immun. 72: 3489-3494 [Abstract] [Full Text]  
• Gong, K., Mailloux, L., Herzberg, M. C. (2000). Salivary Film Expresses a Complex, Macromolecular Binding Site for Streptococcus sanguis. J. Biol. Chem. 275: 8970-8974 [Abstract] [Full Text]  
• Gong, K., Ouyang, T., Herzberg, M. C. (1998). A Streptococcal Adhesion System for Salivary Pellicle and Platelets. Infect. Immun. 66: 5388-5392 [Abstract] [Full Text]