This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Darfeuille-Michaud, A Articles by Cluzel, R Search for Related Content PubMed PubMed Citation Articles by Darfeuille-Michaud, A Articles by Cluzel, R

Identification of a nonfimbrial adhesive factor of an enterotoxigenic Escherichia coli strain.

ABSTRACT

An enterotoxigenic Escherichia coli strain (strain 2230), isolated from a patient with acute infantile diarrhea, was found to adhere only to the brush border of human intestinal epithelial cells. This strain does not hemagglutinate human, bovine, chicken, or guinea pig erythrocytes. The adhesion of E. coli 2230 appears to be mediated by a nonfimbrial bacterial surface protein of 16,000 daltons which can be extracted by heating the bacteria at 60 degrees C for 20 min. This surface protein is implicated as an adhesive factor because pretreatment of enterocytes with this protein extract completely inhibits the adhesion of E. coli 2230. This adhesive factor is serologically distinct from other adhesive factors found in enterotoxigenic E. coli strains. A plasmid DNA of 66 megadaltons is involved in the synthesis of this nonfimbrial adhesive factor.

This article has been cited by other articles:

• Khalil, S. B., Cassels, F. J., Shaheen, H. I., Pannell, L. K., El-Ghorab, N., Kamal, K., Mansour, M., Savarino, S. J., Peruski, L. F. Jr. (1999). Characterization of an Enterotoxigenic Escherichia coli Strain from Africa Expressing a Putative Colonization Factor. Infect. Immun. 67: 4019-4026 [Abstract] [Full Text]  
• Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]