This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Urisu, A Articles by Manclark, C R Search for Related Content PubMed PubMed Citation Articles by Urisu, A Articles by Manclark, C R

 Previous Article  |  Next Article 

Infect Immun. 1986 June; 52(3): 695-701

Filamentous hemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells.

ABSTRACT

[35S]methionine-labeled Bordetella pertussis adhered to monolayers of WiDr cells, an epitheliumlike cell line from a human intestinal carcinoma. Adherence was proportional to the density of the WiDr cells and to the concentration of B. pertussis in the assay. Adherence of virulent phase I strains Tohama phase I, 114, and BP338 was much greater than adherence of avirulent strains Tohama phase III and 423 phase IV. Mutants deficient in the production of the filamentous hemagglutinin (FHA) were hemagglutination negative and adhered to WiDr cells much less efficiently than the parent strains. Preincubation of B. pertussis cells with FHA increased their hemagglutination activity and adherence to WiDr cells. Goat antibody to FHA inhibited, in a dose-dependent manner, the adherence of strain Tohama I but not the adherence of FHA-deficient mutant Tohama 325. At similar protein concentrations, normal goat antibody, goat antibody to pertussis toxin, or the Fab fragments of goat antibody to serotype 2 fimbriae had no effect on adherence. Also, an FHA-positive strain without fimbriae showed high adherence, while a fimbriated FHA-deficient mutant adhered poorly. Our data indicate that FHA plays a major role in adherence of B. pertussis to human WiDr cells. Fimbriae do not appear to mediate attachment of B. pertussis to WiDr cells.

This article has been cited by other articles:

• Choi, P. S., Dawson, A. J., Bernstein, H. D. (2007). Characterization of a Novel Two-Partner Secretion System in Escherichia coli O157:H7. J. Bacteriol. 189: 3452-3461 [Abstract] [Full Text]  
• Inatsuka, C. S., Julio, S. M., Cotter, P. A. (2005). Bordetella filamentous hemagglutinin plays a critical role in immunomodulation, suggesting a mechanism for host specificity. Proc. Natl. Acad. Sci. USA 102: 18578-18583 [Abstract] [Full Text]  
• Elomaa, A., Advani, A., Donnelly, D., Antila, M., Mertsola, J., Hallander, H., He, Q. (2005). Strain Variation among Bordetella pertussis Isolates in Finland, Where the Whole-Cell Pertussis Vaccine Has Been Used for 50 Years. J. Clin. Microbiol. 43: 3681-3687 [Abstract] [Full Text]  
• Jacob-Dubuisson, F., Kehoe, B., Willery, E., Reveneau, N., Locht, C., Relman, D. A. (2000). Molecular characterization of Bordetella bronchiseptica filamentous haemagglutinin and its secretion machinery. Microbiology 146: 1211-1221 [Abstract] [Full Text]  
• van den Berg, B. M., Beekhuizen, H., Willems, R. J. L., Mooi, F. R., van Furth, R. (1999). Role of Bordetella pertussis Virulence Factors in Adherence to Epithelial Cell Lines Derived from the Human Respiratory Tract. Infect. Immun. 67: 1056-1062 [Abstract] [Full Text]  
• Ward, C. K., Lumbley, S. R., Latimer, J. L., Cope, L. D., Hansen, E. J. (1998). Haemophilus ducreyi Secretes a Filamentous Hemagglutinin-Like Protein. J. Bacteriol. 180: 6013-6022 [Abstract] [Full Text]  
• Isberg, R. (1991). Discrimination between intracellular uptake and surface adhesion of bacterial pathogens. Science 252: 934-938 [Abstract]