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Infect Immun. 1986 August; 53(2): 435-437

Composition of affinity-purified alpha-hemolysin of Escherichia coli.

G A Bohach and I S Snyder

ABSTRACT

Escherichia coli alpha-hemolysin was purified from culture supernatants by affinity chromatography, using a hemolysis-neutralizing monoclonal antibody ligand. Purified hemolysin contains several proteins and lipopolysaccharides. Thus, alpha-hemolysin exists as a macromolecular complex and may be exported from E. coli cells by outer membrane fragmentation.


Infect Immun. 1986 August; 53(2): 435-437




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