This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Richardson, K Articles by Kaper, J B Search for Related Content PubMed PubMed Citation Articles by Richardson, K Articles by Kaper, J B

Hemolysin production and cloning of two hemolysin determinants from classical Vibrio cholerae.

ABSTRACT

The hemolytic activity of 20 classical and 3 El Tor strains of V. cholerae O1 was examined phenotypically and genetically. The El Tor strains lysed bovine, chicken, human, rabbit, and sheep erythrocytes (RBCs), while the classical strains lysed only chicken and rabbit RBCs. The assay was done with RBCs in Tris-NaCl buffer, since phosphate-buffered saline was found to inhibit hemolytic activity. Hemolytic activity in culture supernatants from El Tor strains was more sensitive to heat inactivation than that in supernatants from the classical strain 395. A gene library of strain 395 was examined for hemolytic activity, and two distinct hemolytic clones were identified. One clone appeared identical to the previously cloned hemolysin structural gene from El Tor V. cholerae, while the other did not hybridize to the El Tor hemolysin probe, had a unique restriction enzyme digestion pattern, and encoded a hemolysin whose activity differed from that of the El Tor hemolysin clones. We suggest that the hemolysin specified by the determinant originally cloned from an El Tor vibrio be designated hemolysin I and the second hemolysin, cloned from the classical vibrio, be designated hemolysin II.

This article has been cited by other articles:

• Yoon, S. S., Mekalanos, J. J. (2006). 2,3-Butanediol Synthesis and the Emergence of the Vibrio cholerae El Tor Biotype. Infect. Immun. 74: 6547-6556 [Abstract] [Full Text]  
• Coelho, A., Andrade, J. R. C., Vicente, A. C. P., Dirita, V. J. (2000). Cytotoxic Cell Vacuolating Activity from Vibrio cholerae Hemolysin. Infect. Immun. 68: 1700-1705 [Abstract] [Full Text]  
• Pal, S., Datta, A., Nair, G. B., Guhathakurta, B. (1998). Use of Monoclonal Antibodies To Identify Phospholipase C as the Enterotoxic Factor of the Bifunctional Hemolysin-Phospholipase C Molecule of Vibrio cholerae O139. Infect. Immun. 66: 3974-3977 [Abstract] [Full Text]