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Infect Immun. 1987 January; 55(1): 227-232

Isolation of Coccidioides immitis F antigen by immunoaffinity chromatography with monospecific antiserum.

R A Cox, L A Britt and R A Michael

ABSTRACT

Detection of antibody to Coccidioides immitis F antigen is of proved value in the diagnosis of coccidioidomycosis. This antibody is demonstrable by use of an immunodiffusion assay with reference coccidioidin antigen and antiserum to C. immitis. Using a combination of lectin affinity and immunoaffinity chromatography, we isolated the F antigen from coccidioidin and prepared monospecific antibody to the purified antigen. The availability of these reagents will enable the development of a sensitive and specific assay for detecting serologic reactivity to this antigen.

Infect Immun. 1987 January; 55(1): 227-232






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