This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Winkler, J R Articles by Murray, P A Search for Related Content PubMed PubMed Citation Articles by Winkler, J R Articles by Murray, P A

 Previous Article  |  Next Article 

Infect Immun. 1987 November; 55(11): 2721-2726

Attachment of oral bacteria to a basement-membrane-like matrix and to purified matrix proteins.

Department of Stomatology, School of Dentistry, University of California, San Francisco 94143.

ABSTRACT

The purpose of this study was to investigate the adherence of oral bacteria to an in vitro basement-membrane-like matrix and to selected individual macromolecular constituents of this matrix. Radiolabeled bacteria were incubated with basement-membrane-like matrices isolated from PF HR-9 cells. Bacteroides gingivalis 33277, Fusobacterium nucleatum FN-2, and Actinobacillus actinomycetemcomitans GA3(A) bound to the matrix in the range of 44 to 70%, considerably higher than the ranges of A. actinomycetemcomitans GA3(NA) and SUNY AB67 (range, 20 to 25%). The attachment of selected strains of gram-positive bacteria such as Streptococcus and Actinomyces spp. was much less frequent (range, 6 to 25%). Competitive inhibition studies demonstrated that preincubating the bacteria with fibronectin significantly decreased the binding of B. gingivalis by 51% but increased the binding of other gram-negative and gram-positive organisms tested. Similarly, preincubating the matrices with antifibronectin antibodies decreased the binding of B. gingivalis by 31%, whereas the other bacteria tested were either unaffected or binding was increased. The adherence of bacteria to purified basement membrane proteins was also investigated. Strain and species differences were seen in binding, but no clear relationship emerged between binding to an intact matrix and binding to isolated matrix proteins. The results of this study suggest that some gram-negative oral bacteria commonly associated with periodontal disease, such as B. gingivalis, A. actinomycetemcomitans, and F. nucleatum, bound in high numbers to basement-membrane-like matrices in vitro. On the other hand, the gram-positive strains tested bound in much fewer numbers. The results suggest that further studies with this in vitro model may aid in understanding the mechanisms by which oral bacteria adhere to basement membranes.

This article has been cited by other articles:

• Tang, G., Kitten, T., Munro, C. L., Wellman, G. C., Mintz, K. P. (2008). EmaA, a Potential Virulence Determinant of Aggregatibacter actinomycetemcomitans in Infective Endocarditis. Infect. Immun. 76: 2316-2324 [Abstract] [Full Text]  
• Han, Y. W., Ikegami, A., Rajanna, C., Kawsar, H. I., Zhou, Y., Li, M., Sojar, H. T., Genco, R. J., Kuramitsu, H. K., Deng, C. X. (2005). Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria. J. Bacteriol. 187: 5330-5340 [Abstract] [Full Text]  
• Uitto, V.-J., Baillie, D., Wu, Q., Gendron, R., Grenier, D., Putnins, E. E., Kanervo, A., Firth, J. D. (2005). Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells. Infect. Immun. 73: 1171-1179 [Abstract] [Full Text]  
• Gendron, R., Plamondon, P., Grenier, D. (2004). Binding of Pro-Matrix Metalloproteinase 9 by Fusobacterium nucleatum subsp. nucleatum as a Mechanism To Promote the Invasion of a Reconstituted Basement Membrane. Infect. Immun. 72: 6160-6163 [Abstract] [Full Text]